3D in vitro hydrogel models to study the human lung extracellular matrix and fibroblast function

The pulmonary extracellular matrix (ECM) is a macromolecular structure that provides mechanical support, stability and elastic recoil for different pulmonary cells including the lung fibroblasts. The ECM plays an important role in lung development, remodeling, repair, and the maintenance of tissue h...

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Bibliographic Details
Published in:Respiratory research Vol. 24; no. 1; pp. 242 - 15
Main Authors: Phogat, Sakshi, Thiam, Fama, Al Yazeedi, Safiya, Abokor, Filsan Ahmed, Osei, Emmanuel Twumasi
Format: Journal Article
Language:English
Published: London BioMed Central 05.10.2023
BioMed Central Ltd
Nature Publishing Group
BMC
Subjects:
3D hydrogels
Analysis
Asthma
Biomechanics
Care and treatment
Cell culture
Chronic obstructive pulmonary disease
Collagen
Connective tissue
Detergents
Diagnosis
ECM stiffness
Extracellular matrix
Extracellular matrix (ECM)
Extracellular Matrix - metabolism
Fibroblasts
Fibroblasts - metabolism
Fibronectin
Fibrosis
Gels (Pharmacy)
Genotype & phenotype
Health aspects
Homeostasis
Humans
Hyaluronic acid
Hydrogels
Hydrogels - analysis
Hydrogels - metabolism
Idiopathic Pulmonary Fibrosis - metabolism
Lung - metabolism
Lung diseases
Lung fibroblasts
Macromolecules
Medicine
Medicine & Public Health
Molecular structure
Obstructive lung disease
Phenotypes
Pneumology/Respiratory System
Polymers
Proteins
Repair
Respiratory diseases
Review
Three dimensional models
Canada
Extracellular matrix (ECM)
Lung fibroblasts
ECM stiffness
3D hydrogels
Fibrosis
ISSN:1465-993X, 1465-9921, 1465-993X
Online Access:Get full text
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Summary:The pulmonary extracellular matrix (ECM) is a macromolecular structure that provides mechanical support, stability and elastic recoil for different pulmonary cells including the lung fibroblasts. The ECM plays an important role in lung development, remodeling, repair, and the maintenance of tissue homeostasis. Biomechanical and biochemical signals produced by the ECM regulate the phenotype and function of various cells including fibroblasts in the lungs. Fibroblasts are important lung structural cells responsible for the production and repair of different ECM proteins (e.g., collagen and fibronectin). During lung injury and in chronic lung diseases such as asthma, idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD), an abnormal feedback between fibroblasts and the altered ECM disrupts tissue homeostasis and leads to a vicious cycle of fibrotic changes resulting in tissue remodeling. In line with this, using 3D hydrogel culture models with embedded lung fibroblasts have enabled the assessment of the various mechanisms involved in driving defective (fibrotic) fibroblast function in the lung’s 3D ECM environment. In this review, we provide a summary of various studies that used these 3D hydrogel models to assess the regulation of the ECM on lung fibroblast phenotype and function in altered lung ECM homeostasis in health and in chronic respiratory disease.
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ISSN:1465-993X
1465-9921
1465-993X
DOI:10.1186/s12931-023-02548-6