Identification of circulating monocytes as producers of tuberculosis disease biomarker C1q

Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a speci...

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Bibliographic Details
Published in:Scientific Reports Vol. 13; no. 1; pp. 11617 - 10
Main Authors: Niewold, P., Dijkstra, D.J., Cai, Y., Goletti, D., Palmieri, F., Meijgaarden, K.E. van, Verreck, F.A.W., Akkerman, O.W., Hofland, R.W., Delemarre, E.M., Nierkens, S., Verheul, M.K., Pollard, A.J., Dissel, J.T. van, Ottenhoff, T.H.M., Trouw, L.A., Joosten, S.A.
Format: Journal Article
Language:English
Published: London Springer Science and Business Media LLC 18.07.2023
Nature Publishing Group UK
Nature Publishing Group
Nature Portfolio
Subjects:
631/250/255/1856
631/250/590
692/53
Animals
Biomarkers
Biomarkers/metabolism
Complement C1q
Complement C1q/metabolism
Complement component C1q
Disease prevention
Flow cytometry
Health care
Humanities and Social Sciences
Humans
Immunology
Infections
Infectious diseases
Laboratory animals
Medicine
Monkeys & apes
Monocytes
Monocytes/metabolism
multidisciplinary
Primates
Proteins
Public health
Q
R
R&D
Research & development
Research ethics
Research Support, Non-U.S. Gov't
Salmonella
Science
Science (multidisciplinary)
Tuberculosis
Tuberculosis/diagnosis
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-38889-x