Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility

Background African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics a...

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Vydáno v:BMC genomics Ročník 10; číslo 1; s. 207
Hlavní autoři: O'Gorman, Grace M, Park, Stephen DE, Hill, Emmeline W, Meade, Kieran G, Coussens, Paul M, Agaba, Morris, Naessens, Jan, Kemp, Stephen J, MacHugh, David E
Médium: Journal Article
Jazyk:angličtina
Vydáno: London BioMed Central 01.05.2009
BioMed Central Ltd
BMC
Témata:
Animal Genetics and Genomics
Animals
B-Lymphocytes - immunology
Biomedical and Life Sciences
Cattle - genetics
Cattle - immunology
Cattle Diseases - genetics
Cattle Diseases - immunology
Causes of
Diseases
Female
Gene Expression Profiling - veterinary
Genetic aspects
Genetic transcription
Genomics
Immunity, Innate
Life Sciences
Microarrays
Microbial Genetics and Genomics
Oligonucleotide Array Sequence Analysis
Plant Genetics and Genomics
Polymerase chain reaction
Proteomics
Research Article
Risk factors
RNA, Messenger
RNA, Mitochondrial
Sequence Analysis, DNA
Time Factors
Trypanosoma
Trypanosoma congolense
Trypanosomiasis
Trypanosomiasis, African - genetics
Trypanosomiasis, African - immunology
Trypanosomiasis, African - veterinary
Ireland
Boran Group
African Animal Trypanosomiasis
Peripheral Blood Mononuclear Cell
International Livestock Research Institute
Trypanosome Infection
ISSN:1471-2164, 1471-2164
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Shrnutí:Background African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics approach can be used to identify temporal changes in host peripheral blood mononuclear cell (PBMC) gene expression due to disease progression. We also show that major gene expression differences exist between cattle from trypanotolerant and trypanosusceptible breeds. Using bovine long oligonucleotide microarrays and real time quantitative reverse transcription PCR (qRT-PCR) validation we analysed PBMC gene expression in naïve trypanotolerant and trypanosusceptible cattle experimentally challenged with Trypanosoma congolense across a 34-day infection time course. Results Trypanotolerant N'Dama cattle displayed a rapid and distinct transcriptional response to infection, with a ten-fold higher number of genes differentially expressed at day 14 post-infection compared to trypanosusceptible Boran cattle. These analyses identified coordinated temporal gene expression changes for both breeds in response to trypanosome infection. In addition, a panel of genes were identified that showed pronounced differences in gene expression between the two breeds, which may underlie the phenomena of trypanotolerance and trypanosusceptibility. Gene ontology (GO) analysis demonstrate that the products of these genes may contribute to increased mitochondrial mRNA translational efficiency, a more pronounced B cell response, an elevated activation status and a heightened response to stress in trypanotolerant cattle. Conclusion This study has revealed an extensive and diverse range of cellular processes that are altered temporally in response to trypanosome infection in African cattle. Results indicate that the trypanotolerant N'Dama cattle respond more rapidly and with a greater magnitude to infection compared to the trypanosusceptible Boran cattle. Specifically, a subset of the genes analyzed by real time qRT-PCR, which display significant breed differences, could collectively contribute to the trypanotolerance trait in N'Dama.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1471-2164
1471-2164
DOI:10.1186/1471-2164-10-207