Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples

Until recently, the method of choice to characterize viral diversity consisted in cloning PCR amplicons of full-length viral genomes and Sanger-sequencing of multiple clones. However, this is extremely laborious, time-consuming, and low-throughput. Next generation short-read sequencing appears also...

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Vydané v:	PloS one Ročník 13; číslo 3; s. e0194366
Hlavní autori:	Sauvage, Virginie, Boizeau, Laure, Candotti, Daniel, Vandenbogaert, Mathias, Servant-Delmas, Annabelle, Caro, Valérie, Laperche, Syria
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 22.03.2018 Public Library of Science (PLoS)
Predmet:	Biology and Life Sciences Cloning Consortia Deoxyribonucleic acid DNA Ebola virus Ebolavirus Gene expression Gene sequencing Genetic diversity Genome, Viral - genetics Genomes Hepatitis Hepatitis B Hepatitis B - blood Hepatitis B - diagnosis Hepatitis B - virology Hepatitis B virus Hepatitis B virus - genetics High-Throughput Nucleotide Sequencing - methods Humans Infections Laboratories Life Sciences Medicine and health sciences Methods Microbiology and Parasitology Mutation Nanopores New technology Pathogens Polymerase Chain Reaction Polymorphism Polymorphism, Single Nucleotide - genetics Population Porosity Recombination, Genetic - genetics Research and Analysis Methods Sequence Analysis, DNA - methods Technology Technology assessment Viral infections Viral Proteins - genetics Virology Viruses France
ISSN:	1932-6203, 1932-6203
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