Sucrose transporter LeSUT1 and LeSUT2 inhibition affects tomato fruit development in different ways

Sucrose transporters of higher plants belong to a large gene family. At least four different sucrose transporters are known in Solanaceous plants, although their function remains to be elucidated in detail. The isolation of LeSUT1 and LeSUT2 from Lycopersicon esculentum has been described earlier. W...

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Vydané v:	The Plant journal : for cell and molecular biology Ročník 45; číslo 2; s. 180 - 192
Hlavní autori:	Hackel, Aleksandra, Schauer, Nicolas, Carrari, Fernando, Fernie, Alisdair R, Grimm, Bernhard, Kühn, Christina
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Oxford, UK Oxford, UK : Blackwell Science Ltd 01.01.2006 Blackwell Science Ltd Blackwell Science Blackwell Publishing Ltd
Predmet:	Agronomy. Soil science and plant productions antagonists & inhibitors Base Sequence Biological and medical sciences DNA Primers Economic plant physiology Fertility Fructification, ripening. Postharvest physiology fruiting Fruits Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant genes genetics growth & development Growth and development Immunohistochemistry Lycopersicon esculentum Lycopersicon esculentum - genetics Lycopersicon esculentum - growth & development Membrane Transport Proteins Membrane Transport Proteins - genetics Membrane Transport Proteins - physiology metabolic profile Phenotypes phloem phloem loading phloem unloading Physiology Plant Proteins Plant Proteins - antagonists & inhibitors Plant Proteins - genetics Plant Proteins - physiology Plants, Genetically Modified Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Pollen pollen germination pollen tubes Protein Isoforms Protein Isoforms - antagonists & inhibitors Protein Isoforms - genetics Protein Isoforms - physiology seed and fruit development Solanaceae Solanum lycopersicum Solanum lycopersicum var. lycopersicum sucrose sucrose transport Tomatoes transgenic plants transporters pollen germination Seeds Fruit Growth Germination sucrose transport seed and fruit development Pollen tube Phloem Gene phloem unloading Dicotyledones Angiospermae Lycopersicon esculentum metabolic profile Development Spermatophyta Transgenic plant Pollen Solanaceae Inhibition
ISSN:	0960-7412, 1365-313X
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Shrnutí:	Sucrose transporters of higher plants belong to a large gene family. At least four different sucrose transporters are known in Solanaceous plants, although their function remains to be elucidated in detail. The isolation of LeSUT1 and LeSUT2 from Lycopersicon esculentum has been described earlier. Whereas SUT1 is supposed to be the main phloem loader of sucrose in Solanaceae, the role of SUT2 remains a matter of debate. A transgenic approach was taken to evaluate the potential functions of SUT2/SUC3 proteins in sucrose transport or sensing. Expression of LeSUT1 and LeSUT2 was inhibited independently in transgenic tomato plants, using the antisense technique, in order to analyse their specific functions. Although the phloem-specific inhibition of LeSUT1 antisense plants showed a phenotype consistent with an essential role in phloem loading, constitutive LeSUT2 antisense inhibition exclusively affected tomato fruit and seed development. Neither LeSUT1, nor the LeSUT2 antisense plants were able to produce normal tomato fruits; however, it is likely that independent mechanisms underlie these phenomena. While phloem loading was blocked in LeSUT1 antisense plants, the fertility of fruits was reduced in LeSUT2 antisense plants. A detailed physiological analysis of these plants established a role for SUT2 in pollen tube growth and thus assigned a physiological role for SUT2.
Bibliografia:	http://dx.doi.org/10.1111/j.1365-313X.2005.02572.x ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
ISSN:	0960-7412 1365-313X
DOI:	10.1111/j.1365-313X.2005.02572.x