Antigen-Engaged B Cells Undergo Chemotaxis toward the T Zone and Form Motile Conjugates with Helper T Cells

Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boun...

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Bibliographic Details
Published in:PLoS biology Vol. 3; no. 6; p. e150
Main Authors: Okada, Takaharu, Miller, Mark J, Parker, Ian, Krummel, Matthew F, Neighbors, Margaret, Hartley, Suzanne B, O'Garra, Anne, Cahalan, Michael D, Cyster, Jason G
Format: Journal Article
Language:English
Published: United States Public Library of Science 01.06.2005
Public Library of Science (PLoS)
Subjects:
B-Lymphocytes - immunology
Cell Communication - immunology
Chemotaxis, Leukocyte - immunology
Humans
Immune system
Immunology
Lymph nodes
Lymphocytes
Mus (Mouse)
Ovalbumin - immunology
Proteins
Receptors, CCR7
Receptors, Chemokine - immunology
T cell receptors
T-Lymphocytes, Helper-Inducer - immunology
Chemotaxis, Leukocyte
Ovalbumin
Humans
Receptors, CCR7
B-Lymphocytes
Cell Communication
T-Lymphocytes, Helper-Inducer
Receptors, Chemokine
ISSN:1545-7885, 1544-9173, 1545-7885
Online Access:Get full text
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Summary:Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boundary in a CCR7-dependent manner, through a region that exhibits a CCR7-ligand gradient. Initially the B cells show reduced motility, but after 1 d, motility is increased to approximately 9 microm/min. Antigen-engaged B cells pair with antigen-specific helper T cells for 10 to more than 60 min, whereas non-antigen-specific interactions last less than 10 min. B cell-T cell conjugates are highly dynamic and migrate extensively, being led by B cells. B cells occasionally contact more than one T cell, whereas T cells are strictly monogamous in their interactions. These findings provide evidence of lymphocyte chemotaxis in vivo, and they begin to define the spatiotemporal cellular dynamics associated with T cell-dependent antibody responses.
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ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.0030150