Monoclonal antibodies to Cache Valley virus for serological diagnosis

Cache Valley virus (CVV) is a mosquito-borne virus in the genus Orthobunyavirus , family Peribunyaviridae . It was first isolated from a Culiseta inorata mosquito in Cache Valley, Utah in 1956 and is known to circulate widely in the Americas. While only a handful of human cases have been reported si...

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Vydané v:	PLoS neglected tropical diseases Ročník 16; číslo 1; s. e0010156
Hlavní autori:	Skinner, Benjamin, Mikula, Sierra, Davis, Brent S., Powers, Jordan A., Hughes, Holly R., Calvert, Amanda E.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Public Library of Science 01.01.2022 Public Library of Science (PLoS)
Predmet:	Animals Antibodies Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Aquatic insects Arbovirus diseases Biology and life sciences Bunyamwera virus - immunology Bunyaviridae Infections - diagnosis Bunyaviridae Infections - virology Cell Line Chlorocebus aethiops Clones Cloning Cross Reactions - immunology Cross-reactivity Diagnosis Diagnostic systems Disease Disease Models, Animal Distribution Enzyme-Linked Immunosorbent Assay Enzymes Fetuses Genomes Glycoproteins Health aspects Humans Immunization Immunoassay Immunoassays Immunoglobulin M Infections Livestock Livestock - virology Medical laboratories Medicine and health sciences Mice Mice, Knockout Monoclonal antibodies Mosquitoes Multiple myeloma Neutralization Nucleocapsid Proteins - immunology Pathogens Playas Proteins Research and Analysis Methods RNA polymerase Sensitivity and Specificity Serologic Tests Serology Sheep Tropical diseases Valleys Vector Borne Diseases - virology Vero Cells Viruses United States Vancouver British Columbia Canada Canada United States > US
ISSN:	1935-2735, 1935-2727, 1935-2735
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Popis
Shrnutí:	Cache Valley virus (CVV) is a mosquito-borne virus in the genus Orthobunyavirus , family Peribunyaviridae . It was first isolated from a Culiseta inorata mosquito in Cache Valley, Utah in 1956 and is known to circulate widely in the Americas. While only a handful of human cases have been reported since its discovery, it is the causative agent of fetal death and severe malformations in livestock. CVV has recently emerged as a potential viral pathogen causing severe disease in humans. Currently, the only serological assay available for diagnostic testing is plaque reduction neutralization test which takes several days to perform and requires biocontainment. To expand diagnostic capacity to detect CVV infections by immunoassays, 12 hybridoma clones secreting anti-CVV murine monoclonal antibodies (MAbs) were developed. All MAbs developed were found to be non-neutralizing and specific to the nucleoprotein of CVV. Cross-reactivity experiments with related orthobunyaviruses revealed several of the MAbs reacted with Tensaw, Fort Sherman, Tlacotalpan, Maguari, Playas, and Potosi viruses. Our data shows that MAbs CVV14, CVV15, CVV17, and CVV18 have high specific reactivity as a detector in an IgM antibody capture test with human sera.
Bibliografia:	new_version ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 The authors have declared that no competing interests exist.
ISSN:	1935-2735 1935-2727 1935-2735
DOI:	10.1371/journal.pntd.0010156