2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting

Background Proteomic technologies applied for profiling human biofluids and blood cells are considered to reveal new biomarkers of exposure or provide insights into novel mechanisms of adaptation. Methods Both a non-targeted (classical 2D-electrophoresis combined with mass spectrometry) as well as a...

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Vydáno v:BMC medical genomics Ročník 4; číslo 1; s. 24
Hlavní autoři: Bouwman, Freek G, de Roos, Baukje, Rubio-Aliaga, Isabel, Crosley, L Katie, Duthie, Susan J, Mayer, Claus, Horgan, Graham, Polley, Abigael C, Heim, Carolin, Coort, Susan LM, Evelo, Chris T, Mulholland, Francis, Johnson, Ian T, Elliott, Ruan M, Daniel, Hannelore, Mariman, Edwin CM
Médium: Journal Article
Jazyk:angličtina
Vydáno: London BioMed Central 25.03.2011
BioMed Central Ltd
Springer Nature B.V
BMC
Témata:
Apolipoproteins A - blood
Biological markers
Biological variation
Biomarkers
Biomarkers - metabolism
Biomedical and Life Sciences
Biomedicine
Blood
Blood Cells - metabolism
Blood platelets
Body fluids
Body Fluids - metabolism
Cluster Analysis
Design
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Experiments
Fasting
Gene Expression
Guanine Nucleotide Dissociation Inhibitors - metabolism
Human Genetics
Humans
Immunoassay
Leptin - metabolism
Mass Spectrometry
Matrix Metalloproteinase 3 - metabolism
Medical research
Microarrays
Nutrition
Nutrition research
Physiological aspects
Plasma
Principal Component Analysis
Prognostics and diagnostics/biomarkers
Proteins
Proteome - metabolism
Proteomics
Proteomics - methods
Research Article
Research parks
rho Guanine Nucleotide Dissociation Inhibitor beta
rho-Specific Guanine Nucleotide Dissociation Inhibitors
Time Factors
Tumor Suppressor Proteins - metabolism
United Kingdom
Netherlands
Germany
Random Forest Analysis
Multiplex Immunoassay
Peripheral Blood Mononuclear Cell
Random Forest
MALDI Target Plate
ISSN:1755-8794, 1755-8794
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Shrnutí:Background Proteomic technologies applied for profiling human biofluids and blood cells are considered to reveal new biomarkers of exposure or provide insights into novel mechanisms of adaptation. Methods Both a non-targeted (classical 2D-electrophoresis combined with mass spectrometry) as well as a targeted proteomic approach (multiplex immunoassay) were applied to investigate how fasting for 36 h, as compared to 12 h, affects the proteome of platelets, peripheral blood mononuclear cells (PBMC), plasma, urine and saliva collected from ten healthy volunteers. Results Between-subject variability was highest in the plasma proteome and lowest in the PBMC proteome. Random Forests analysis performed on the entire dataset revealed that changes in the level of the RhoGDI2 protein in PBMC and plasma ApoA4 levels were the two most obvious biomarkers of an extended fasting. Random Forests (RF) analysis of the multiplex immunoassay data revealed leptin and MMP-3 as biomarkers for extended fasting. However, high between-subject variability may have masked the extended fasting effects in the proteome of the biofluids and blood cells. Conclusions Identification of significantly changed proteins in biofluids and blood cells using a non-targeted approach, together with the outcome of targeted analysis revealed both known and novel markers for a 36 h fasting period, including the cellular proteins RhoGDI2 and CLIC1, and plasma proteins ApoA4, leptin and MMP-3. The PBMC proteome exhibited the lowest between-subject variability and therefore these cells appear to represent the best biosamples for biomarker discovery in human nutrigenomics.
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ISSN:1755-8794
1755-8794
DOI:10.1186/1755-8794-4-24