In vivo imaging of immediate early gene expression reveals layer-specific memory traces in the mammalian brain

The dynamic processes of formatting long-term memory traces in the cortex are poorly understood. The investigation of these processes requires measurements of task-evoked neuronal activities from large numbers of neurons over many days. Here, we present a two-photon imaging-based system to track eve...

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Vydáno v:Proceedings of the National Academy of Sciences - PNAS Ročník 111; číslo 7; s. 2788
Hlavní autoři: Xie, Hong, Liu, Yu, Zhu, Youzhi, Ding, Xinlu, Yang, Yuhao, Guan, Ji-Song
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 18.02.2014
Témata:
Animals
Brain Mapping - methods
Cerebral Cortex - cytology
Cerebral Cortex - physiology
Gene Expression - physiology
Genes, Immediate-Early - genetics
Genes, Immediate-Early - physiology
Green Fluorescent Proteins - metabolism
Image Processing, Computer-Assisted
Memory, Long-Term - physiology
Mice
Microscopy, Fluorescence
Neurons - metabolism
Neurons - physiology
ISSN:1091-6490, 1091-6490
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Shrnutí:The dynamic processes of formatting long-term memory traces in the cortex are poorly understood. The investigation of these processes requires measurements of task-evoked neuronal activities from large numbers of neurons over many days. Here, we present a two-photon imaging-based system to track event-related neuronal activity in thousands of neurons through the quantitative measurement of EGFP proteins expressed under the control of the EGR1 gene promoter. A recognition algorithm was developed to detect GFP-positive neurons in multiple cortical volumes and thereby to allow the reproducible tracking of 4,000 neurons in each volume for 2 mo. The analysis revealed a context-specific response in sparse layer II neurons. The context-evoked response gradually increased during several days of training and was maintained 1 mo later. The formed traces were specifically activated by the training context and were linearly correlated with the behavioral response. Neuronal assemblies that responded to specific contexts were largely separated, indicating the sparse coding of memory-related traces in the layer II cortical circuit.
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ISSN:1091-6490
1091-6490
DOI:10.1073/pnas.1316808111