SARS-CoV-2 reliably detected in frozen saliva samples stored up to one year

Viability of saliva samples stored for longer than 28 days has not been reported in the literature. The COVID-19 pandemic has spawned new research evaluating various sample types, thus large biobanks have been started. Residual saliva samples from university student surveillance testing were reteste...

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Bibliographic Details
Published in:PloS one Vol. 17; no. 8; p. e0272971
Main Authors: Frediani, Jennifer K., McLendon, Kaleb B., Westbrook, Adrianna, Gillespie, Scott E., Wood, Anna, Baugh, Tyler J., O’Sick, William, Roback, John D., Lam, Wilbur A., Levy, Joshua M.
Format: Journal Article
Language:English
Published: San Francisco Public Library of Science 11.08.2022
Public Library of Science (PLoS)
Subjects:
Antigens
Biology and Life Sciences
Coronaviruses
COVID-19
COVID-19 diagnostic tests
Evaluation
Medicine and Health Sciences
Pandemics
Research and Analysis Methods
Saliva
Salivary diagnostics
Sensitivity analysis
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Social Sciences
United States
United States > US
ISSN:1932-6203, 1932-6203
Online Access:Get full text
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Summary:Viability of saliva samples stored for longer than 28 days has not been reported in the literature. The COVID-19 pandemic has spawned new research evaluating various sample types, thus large biobanks have been started. Residual saliva samples from university student surveillance testing were retested on SalivaDirect and compared with original RT-PCR (cycle threshold values) and quantitative antigen values for each month in storage. We conclude that saliva samples stored at -80°C are still viable in detecting SARS-CoV-2 after 12 months of storage, establishing the validity of these samples for future testing.
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Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0272971