Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale

Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work h...

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Vydáno v:Applied and environmental microbiology Ročník 75; číslo 15; s. 5111
Hlavní autoři: Lauber, Christian L, Hamady, Micah, Knight, Rob, Fierer, Noah
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.08.2009
Témata:
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Biodiversity
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Hydrogen-Ion Concentration
North America
Sequence Analysis, DNA - methods
Soil - analysis
Soil Microbiology
South America
ISSN:1098-5336, 1098-5336
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Shrnutí:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on either surveying a relatively small number of soils in detail or analyzing a larger number of soils with techniques that provide little detail about the phylogenetic structure of the bacterial communities. Here we used a bar-coded pyrosequencing technique to characterize bacterial communities in 88 soils from across North and South America, obtaining an average of 1,501 sequences per soil. We found that overall bacterial community composition, as measured by pairwise UniFrac distances, was significantly correlated with differences in soil pH (r = 0.79), largely driven by changes in the relative abundances of Acidobacteria, Actinobacteria, and Bacteroidetes across the range of soil pHs. In addition, soil pH explains a significant portion of the variability associated with observed changes in the phylogenetic structure within each dominant lineage. The overall phylogenetic diversity of the bacterial communities was also correlated with soil pH (R(2) = 0.50), with peak diversity in soils with near-neutral pHs. Together, these results suggest that the structure of soil bacterial communities is predictable, to some degree, across larger spatial scales, and the effect of soil pH on bacterial community composition is evident at even relatively coarse levels of taxonomic resolution.
Bibliografie:ObjectType-Article-1
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ISSN:1098-5336
1098-5336
DOI:10.1128/AEM.00335-09