Nanocomposite-based PCR reactors to enhance thermal rate and fluorescence intensity in hand-held qPCR device

A photonic quantitative polymerase chain reaction (qPCR) has usually implemented a polydimethylsiloxane (PDMS) based disposable inexpensive PCR reactor, worked as the photothermal cycler, to show potential as a point-of-care test (PoCT) for detection nucleic acids. However, the PoCT type photonic qP...

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Bibliographic Details
Published in:Journal of nanobiotechnology Vol. 23; no. 1; pp. 240 - 13
Main Authors: Han, Jiyeon, A M, Tiara, Kim, Seongryeong, Morales Florez, Gabriela, Shrestha, Kiran, Nguyen, Dang Du, Kim, Inki, Lee, Jinkee, Cho, Gyoujin
Format: Journal Article
Language:English
Published: London BioMed Central 23.03.2025
BioMed Central Ltd
BMC
Subjects:
Biotechnology
Chemical reactors
Chemistry
Chemistry and Materials Science
Diagnostic equipment (Medical)
Dimethylpolysiloxanes - chemistry
Engineering research
Fluorescence
Methods
Molecular Medicine
Nanocomposites - chemistry
Nanoparticles
Nanotechnology
Nucleic acid assay
Photonic PCR
Photothermal cycler
Plasmonic fluorescence
Point-of-Care Systems
Polymerase chain reaction
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Temperature
TiO2-PDMS
Titanium - chemistry
Titanium dioxide
South Korea
Plasmonic fluorescence
Photothermal cycler
Photonic PCR
TiO
Nucleic acid assay
PDMS
TiO2-PDMS
ISSN:1477-3155, 1477-3155
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Summary:A photonic quantitative polymerase chain reaction (qPCR) has usually implemented a polydimethylsiloxane (PDMS) based disposable inexpensive PCR reactor, worked as the photothermal cycler, to show potential as a point-of-care test (PoCT) for detection nucleic acids. However, the PoCT type photonic qPCR has to overcome the prolonged time for the fabrication of PDMS-based PCR reactors and enable a rapid thermal cycler to shorten diagnosis time with a strong fluorescence intensity. Here, we developed a room-temperature curable titanium dioxide (TiO 2 ) nanoparticle dispersed PDMS (TiO 2 -PDMS) nanocomposite to reduce the fabrication time of the PCR reactor which enhanced the speed of photothermal cycles and fluorescence signal intensity of photonic qPCR. The TiO 2 -PDMS nanocomposite was formulated for rapid cross-linking at the room-temperature by introducing an optimized amount of Pt catalyst, resulting in the fabrication of a nanocomposite-based PCR reactor within 8 min at room-temperature. The nanocomposite-based PCR reactor enhanced the heating rate to 18.33 Cº/s and cooling rate to −3.11Cº/s because of the phonon scattering effect of TiO 2 in the reactor and successfully amplified λ-DNA (amplicon size of 100 bp) within 10 min. Finally, we improved the qPCR efficiency by 2 cycle threshold ( C t ) value compared with pristine PDMS reactor and quantified up to 10 copies/µL nucleic acids by fluorescence intensity enhancement resulting from light reflections property of TiO 2 . By using TiO 2 -PDMS nanocomposite-based PCR reactors, the fast and efficient nucleic acid assay was enabled without loss of sensitivity, and it can be practically used in the field of PoCT. Graphical Abstract
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ISSN:1477-3155
1477-3155
DOI:10.1186/s12951-025-03287-0