Nanocomposite-based PCR reactors to enhance thermal rate and fluorescence intensity in hand-held qPCR device

A photonic quantitative polymerase chain reaction (qPCR) has usually implemented a polydimethylsiloxane (PDMS) based disposable inexpensive PCR reactor, worked as the photothermal cycler, to show potential as a point-of-care test (PoCT) for detection nucleic acids. However, the PoCT type photonic qP...

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Vydáno v:	Journal of nanobiotechnology Ročník 23; číslo 1; s. 240 - 13
Hlavní autoři:	Han, Jiyeon, A M, Tiara, Kim, Seongryeong, Morales Florez, Gabriela, Shrestha, Kiran, Nguyen, Dang Du, Kim, Inki, Lee, Jinkee, Cho, Gyoujin
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 23.03.2025 BioMed Central Ltd BMC
Témata:	Biotechnology Chemical reactors Chemistry Chemistry and Materials Science Diagnostic equipment (Medical) Dimethylpolysiloxanes - chemistry Engineering research Fluorescence Methods Molecular Medicine Nanocomposites - chemistry Nanoparticles Nanotechnology Nucleic acid assay Photonic PCR Photothermal cycler Plasmonic fluorescence Point-of-Care Systems Polymerase chain reaction Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Temperature TiO2-PDMS Titanium - chemistry Titanium dioxide South Korea Plasmonic fluorescence Photothermal cycler Photonic PCR TiO Nucleic acid assay PDMS TiO2-PDMS
ISSN:	1477-3155, 1477-3155
On-line přístup:	Získat plný text
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Popis
Shrnutí:	A photonic quantitative polymerase chain reaction (qPCR) has usually implemented a polydimethylsiloxane (PDMS) based disposable inexpensive PCR reactor, worked as the photothermal cycler, to show potential as a point-of-care test (PoCT) for detection nucleic acids. However, the PoCT type photonic qPCR has to overcome the prolonged time for the fabrication of PDMS-based PCR reactors and enable a rapid thermal cycler to shorten diagnosis time with a strong fluorescence intensity. Here, we developed a room-temperature curable titanium dioxide (TiO 2 ) nanoparticle dispersed PDMS (TiO 2 -PDMS) nanocomposite to reduce the fabrication time of the PCR reactor which enhanced the speed of photothermal cycles and fluorescence signal intensity of photonic qPCR. The TiO 2 -PDMS nanocomposite was formulated for rapid cross-linking at the room-temperature by introducing an optimized amount of Pt catalyst, resulting in the fabrication of a nanocomposite-based PCR reactor within 8 min at room-temperature. The nanocomposite-based PCR reactor enhanced the heating rate to 18.33 Cº/s and cooling rate to −3.11Cº/s because of the phonon scattering effect of TiO 2 in the reactor and successfully amplified λ-DNA (amplicon size of 100 bp) within 10 min. Finally, we improved the qPCR efficiency by 2 cycle threshold ( C t ) value compared with pristine PDMS reactor and quantified up to 10 copies/µL nucleic acids by fluorescence intensity enhancement resulting from light reflections property of TiO 2 . By using TiO 2 -PDMS nanocomposite-based PCR reactors, the fast and efficient nucleic acid assay was enabled without loss of sensitivity, and it can be practically used in the field of PoCT. Graphical Abstract
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1477-3155 1477-3155
DOI:	10.1186/s12951-025-03287-0