Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing....

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Bibliographic Details
Published in:	Nature communications Vol. 11; no. 1; pp. 4812 - 12
Main Authors:	Smyrlaki, Ioanna, Ekman, Martin, Lentini, Antonio, Rufino de Sousa, Nuno, Papanicolaou, Natali, Vondracek, Martin, Aarum, Johan, Safari, Hamzah, Muradrasoli, Shaman, Rothfuchs, Antonio Gigliotti, Albert, Jan, Högberg, Björn, Reinius, Björn
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 23.09.2020 Nature Portfolio
Subjects:	38/77 38/88 631/326/596/4130 692/699/255/2514 692/700/139/1420 Benchmarking Betacoronavirus - genetics Betacoronavirus - isolation & purification Clinical Laboratory Techniques - methods Clinical Laboratory Techniques - standards Clinical Laboratory Techniques - statistics & numerical data Coronavirus Infections - diagnosis Coronavirus Infections - epidemiology Coronavirus Infections - virology COVID-19 COVID-19 Testing DNA Primers - genetics Hot Temperature Humanities and Social Sciences Humans multidisciplinary Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - epidemiology Pneumonia, Viral - virology Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - standards Reverse Transcriptase Polymerase Chain Reaction - statistics & numerical data RNA, Viral - genetics RNA, Viral - isolation & purification SARS-CoV-2 Science Science (multidisciplinary) Sensitivity and Specificity Sweden - epidemiology Viral Plaque Assay - methods Sweden
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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