Interchangeability of light and virtual microscopy for histopathological evaluation of prostate cancer

Virtual microscopy (VM) holds promise to reduce subjectivity as well as intra- and inter-observer variability for the histopathological evaluation of prostate cancer. We evaluated (i) the repeatability (intra-observer agreement) and reproducibility (inter-observer agreement) of the 2014 Gleason grad...

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Published in:Scientific reports Vol. 11; no. 1; pp. 3257 - 11
Main Authors: Zelic, Renata, Giunchi, Francesca, Lianas, Luca, Mascia, Cecilia, Zanetti, Gianluigi, Andrén, Ove, Fridfeldt, Jonna, Carlsson, Jessica, Davidsson, Sabina, Molinaro, Luca, Vincent, Per Henrik, Richiardi, Lorenzo, Akre, Olof, Fiorentino, Michelangelo, Pettersson, Andreas
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 05.02.2021
Nature Publishing Group
Nature Portfolio
Subjects:
692/4025/1752
692/4028/67/589/466
692/700/478/174
Agreements
Glands
Humanities and Social Sciences
Light microscopy
Metastases
Microscopy
multidisciplinary
Prostate cancer
Science
Science (multidisciplinary)
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Virtual microscopy (VM) holds promise to reduce subjectivity as well as intra- and inter-observer variability for the histopathological evaluation of prostate cancer. We evaluated (i) the repeatability (intra-observer agreement) and reproducibility (inter-observer agreement) of the 2014 Gleason grading system and other selected features using standard light microscopy (LM) and an internally developed VM system, and (ii) the interchangeability of LM and VM. Two uro-pathologists reviewed 413 cores from 60 Swedish men diagnosed with non-metastatic prostate cancer 1998–2014. Reviewer 1 performed two reviews using both LM and VM. Reviewer 2 performed one review using both methods. The intra- and inter-observer agreement within and between LM and VM were assessed using Cohen’s kappa and Bland and Altman’s limits of agreement. We found good repeatability and reproducibility for both LM and VM, as well as interchangeability between LM and VM, for primary and secondary Gleason pattern, Gleason Grade Groups, poorly formed glands, cribriform pattern and comedonecrosis but not for the percentage of Gleason pattern 4. Our findings confirm the non-inferiority of VM compared to LM. The repeatability and reproducibility of percentage of Gleason pattern 4 was poor regardless of method used warranting further investigation and improvement before it is used in clinical practice.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-82911-z