A membrane protein based biosensor: Use of a phosphate – H + symporter membrane protein (Pho84) in the sensing of phosphate ions

A label free biosensor for direct detection of inorganic phosphate based on potential-step capacitance measurements has been developed. The high-affinity Pho84 plasma membrane phosphate/proton symporter of Saccharomyces cerevisiae was used as a sensing element. Heterologously expressed and purified...

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Bibliographic Details
Published in:	Biosensors & bioelectronics Vol. 27; no. 1; pp. 58 - 63
Main Authors:	Basheer, Shabana, Samyn, Dieter, Hedström, Martin, Thakur, Munna Singh, Persson, Bengt L., Mattiasson, Bo
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier B.V 15.09.2011 Elsevier
Subjects:	Biochemistry Biokemi Biological and medical sciences Biosensing Techniques Biosensors Biotechnology capacitance Capacitive biosensor detection limit Electric Capacitance electrodes Engineering and Technology Fundamental and applied biological sciences. Psychology Immobilized Proteins - chemistry Industrial Biotechnology Industriell bioteknik ions Ions - analysis Membrane protein membrane proteins Membrane Proteins - chemistry Methods. Procedures. Technologies NATURAL SCIENCES NATURVETENSKAP Pho84 Phosphate phosphates Phosphates - analysis plasma membrane Proton-Phosphate Symporters - chemistry protons Saccharomyces Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - chemistry Symporter Teknik Various methods and equipments Capacitive biosensor Symporter Membrane protein Pho84 Phosphate Phosphates Biosensor Membrane Protein
ISSN:	0956-5663, 1873-4235, 1873-4235
Online Access:	Get full text
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