A membrane protein based biosensor: Use of a phosphate – H + symporter membrane protein (Pho84) in the sensing of phosphate ions

A label free biosensor for direct detection of inorganic phosphate based on potential-step capacitance measurements has been developed. The high-affinity Pho84 plasma membrane phosphate/proton symporter of Saccharomyces cerevisiae was used as a sensing element. Heterologously expressed and purified...

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Vydáno v:	Biosensors & bioelectronics Ročník 27; číslo 1; s. 58 - 63
Hlavní autoři:	Basheer, Shabana, Samyn, Dieter, Hedström, Martin, Thakur, Munna Singh, Persson, Bengt L., Mattiasson, Bo
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Kidlington Elsevier B.V 15.09.2011 Elsevier
Témata:	Biochemistry Biokemi Biological and medical sciences Biosensing Techniques Biosensors Biotechnology capacitance Capacitive biosensor detection limit Electric Capacitance electrodes Engineering and Technology Fundamental and applied biological sciences. Psychology Immobilized Proteins - chemistry Industrial Biotechnology Industriell bioteknik ions Ions - analysis Membrane protein membrane proteins Membrane Proteins - chemistry Methods. Procedures. Technologies NATURAL SCIENCES NATURVETENSKAP Pho84 Phosphate phosphates Phosphates - analysis plasma membrane Proton-Phosphate Symporters - chemistry protons Saccharomyces Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - chemistry Symporter Teknik Various methods and equipments Capacitive biosensor Symporter Membrane protein Pho84 Phosphate Phosphates Biosensor Membrane Protein
ISSN:	0956-5663, 1873-4235, 1873-4235
On-line přístup:	Získat plný text
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