Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring

We report a covalent chemistry-based hepatocellular carcinoma (HCC)-specific extracellular vesicle (EV) purification system for early detection of HCC by performing digital scoring on the purified EVs. Earlier detection of HCC creates more opportunities for curative therapeutic interventions. EVs ar...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Nature communications Ročník 11; číslo 1; s. 4489 - 12
Hlavní autoři:	Sun, Na, Lee, Yi-Te, Zhang, Ryan Y., Kao, Rueihung, Teng, Pai-Chi, Yang, Yingying, Yang, Peng, Wang, Jasmine J., Smalley, Matthew, Chen, Pin-Jung, Kim, Minhyung, Chou, Shih-Jie, Bao, Lirong, Wang, Jing, Zhang, Xinyue, Qi, Dongping, Palomique, Juvelyn, Nissen, Nicolas, Han, Steven-Huy B., Sadeghi, Saeed, Finn, Richard S., Saab, Sammy, Busuttil, Ronald W., Markovic, Daniela, Elashoff, David, Yu, Hsiao-hua, Li, Huiying, Heaney, Anthony P., Posadas, Edwin, You, Sungyong, Yang, Ju Dong, Pei, Renjun, Agopian, Vatche G., Tseng, Hsian-Rong, Zhu, Yazhen
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 07.09.2020 Nature Publishing Group Nature Portfolio
Témata:	13/1 13/51 13/62 147/135 147/143 38/39 38/77 49/90 631/1647/350/877 631/67/1504/1610/4029 631/67/2322 692/53/2421 Aged Antibodies Biomarkers, Tumor - genetics Biomarkers, Tumor - isolation & purification Cancer Carcinoma, Hepatocellular - blood Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Case-Control Studies Chemistry Click Chemistry - instrumentation Click Chemistry - methods Computational Chemistry Computer Simulation Covalence Diagnosis, Differential Digital signatures Dimethylpolysiloxanes - chemistry Disease Progression Early Detection of Cancer - instrumentation Early Detection of Cancer - methods Extracellular vesicles Extracellular Vesicles - genetics Female Hep G2 Cells Hepatocellular carcinoma Humanities and Social Sciences Humans Lab-On-A-Chip Devices Liquid Biopsy - instrumentation Liquid Biopsy - methods Liver cancer Liver Cirrhosis - blood Liver Cirrhosis - pathology Liver Neoplasms - blood Liver Neoplasms - diagnosis Liver Neoplasms - genetics Liver Neoplasms - pathology Male Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Middle Aged Mixers multidisciplinary Nanostructures - chemistry Nanowires - chemistry Neoplasm Staging Purification Reverse Transcriptase Polymerase Chain Reaction - instrumentation Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - genetics RNA, Messenger - isolation & purification ROC Curve Science Science (multidisciplinary) Substrates Therapeutic applications Vesicles
ISSN:	2041-1723, 2041-1723
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	We report a covalent chemistry-based hepatocellular carcinoma (HCC)-specific extracellular vesicle (EV) purification system for early detection of HCC by performing digital scoring on the purified EVs. Earlier detection of HCC creates more opportunities for curative therapeutic interventions. EVs are present in circulation at relatively early stages of disease, providing potential opportunities for HCC early detection. We develop an HCC EV purification system (i.e., EV Click Chips) by synergistically integrating covalent chemistry-mediated EV capture/release, multimarker antibody cocktails, nanostructured substrates, and microfluidic chaotic mixers. We then explore the translational potential of EV Click Chips using 158 plasma samples of HCC patients and control cohorts. The purified HCC EVs are subjected to reverse-transcription droplet digital PCR for quantification of 10 HCC-specific mRNA markers and computation of digital scoring. The HCC EV-derived molecular signatures exhibit great potential for noninvasive early detection of HCC from at-risk cirrhotic patients with an area under receiver operator characteristic curve of 0.93 (95% CI, 0.86 to 1.00; sensitivity = 94.4%, specificity = 88.5%). Extracellular vesicles (EVs) are present in circulation at relatively early stages of disease, providing potential opportunities for early cancer diagnosis. Here, the authors report a covalent chemistry-based hepatocellular carcinoma (HCC)-specific EV purification system for early detection of HCC by performing digital scoring on the purified EVs.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Undefined-3
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-020-18311-0