Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features required for replication

Influenza A virus nucleoprotein (NP) association with viral RNA (vRNA) is essential for packaging, but the pattern of NP binding to vRNA is unclear. Here we applied photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation (PAR-CLIP) to assess the native-state of NP–vRNA interac...

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Bibliographic Details
Published in:	Nature communications Vol. 9; no. 1; pp. 465 - 12
Main Authors:	Williams, Graham D., Townsend, Dana, Wylie, Kristine M., Kim, Preston J., Amarasinghe, Gaya K., Kutluay, Sebla B., Boon, Adrianus C. M.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 31.01.2018 Nature Publishing Group Nature Portfolio
Subjects:	14/63 45/70 45/88 45/90 45/91 631/1647/514/2254 631/326/596/1578 Animals Binding Conserved Sequence Crosslinking Dogs Gene mapping Genome, Viral Genomes HEK293 Cells Humanities and Social Sciences Humans Immunoprecipitation Influenza Influenza A Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - metabolism Madin Darby Canine Kidney Cells multidisciplinary Mutagenesis Mutation Nucleotide Mapping Nucleotide sequence Nucleotides Packaging Packaging design Pyridine nucleotide reductase Ribonucleic acid RNA RNA viruses RNA, Viral - genetics RNA, Viral - metabolism RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Science Science (multidisciplinary) Viral Core Proteins - genetics Viral Core Proteins - metabolism Virus Replication - genetics Viruses
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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