Kinetic analysis reveals the fate of a microRNA following target regulation in mammalian cells

Considerable details about microRNA (miRNA) biogenesis and regulation have been uncovered, but little is known about the fate of the miRNA subsequent to target regulation. To gain insight into this process, we carried out kinetic analysis of a miRNA's turnover following termination of its bioge...

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Vydáno v:Current biology Ročník 21; číslo 5; s. 369
Hlavní autoři: Baccarini, Alessia, Chauhan, Hemangini, Gardner, Thomas J, Jayaprakash, Anitha D, Sachidanandam, Ravi, Brown, Brian D
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 08.03.2011
Témata:
Base Sequence
Cell Line
DNA Primers - genetics
Flow Cytometry
Gene Expression Regulation - genetics
Genetic Vectors
Humans
Kinetics
MicroRNAs - genetics
MicroRNAs - physiology
Models, Genetic
Molecular Sequence Data
Nerve Tissue Proteins - genetics
Oligonucleotides - genetics
Receptors, Nerve Growth Factor - genetics
RNA Stability - physiology
Sequence Analysis, DNA
ISSN:1879-0445, 1879-0445
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Shrnutí:Considerable details about microRNA (miRNA) biogenesis and regulation have been uncovered, but little is known about the fate of the miRNA subsequent to target regulation. To gain insight into this process, we carried out kinetic analysis of a miRNA's turnover following termination of its biogenesis and during regulation of a target that is not subject to Ago2-mediated catalytic cleavage. By quantitating the number of molecules of the miRNA and its target in steady state and in the course of its decay, we found that each miRNA molecule was able to regulate at least two target transcripts, providing in vivo evidence that the miRNA is not irreversibly sequestered with its target and that the nonslicing pathway of miRNA regulation is multiple-turnover. Using deep sequencing, we further show that miRNA recycling is limited by target regulation, which promotes posttranscriptional modifications to the 3' end of the miRNA and accelerates the miRNA's rate of decay. These studies provide new insight into the efficiency of miRNA regulation that help to explain how a miRNA can regulate a vast number of transcripts and that identify one of the mechanisms that impart specificity to miRNA decay in mammalian cells.
Bibliografie:ObjectType-Article-1
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ISSN:1879-0445
1879-0445
DOI:10.1016/j.cub.2011.01.067