Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue

Fluorescent antimicrobial peptides are promising structures for in situ , real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage...

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Bibliographic Details
Published in:Nature communications Vol. 7; no. 1; pp. 10940 - 9
Main Authors: Mendive-Tapia, Lorena, Zhao, Can, Akram, Ahsan R., Preciado, Sara, Albericio, Fernando, Lee, Martin, Serrels, Alan, Kielland, Nicola, Read, Nick D, Lavilla, Rodolfo, Vendrell, Marc
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 09.03.2016
Nature Publishing Group
Nature Portfolio
Subjects:
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Amino acids
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Antiinfectives and antibacterials
Antimicrobial peptides
Aspergillosis - diagnosis
Aspergillosis - microbiology
Aspergillus fumigatus - chemistry
Aspergillus fumigatus - drug effects
Aspergillus fumigatus - physiology
Bacterial infections
Boron Compounds - chemistry
Diagnostic Imaging - instrumentation
Diagnostic Imaging - methods
Fluorescence
Fluorescent Dyes - chemistry
Fungal infections
Fungi
Human performance
Human tissues
Humanities and Social Sciences
Humans
Hydrophobicity
Imaging
Labeling
Lung - microbiology
Medical research
Microenvironments
multidisciplinary
Organic chemistry
Peptides
Peptides - chemistry
Peptides - pharmacology
Permeability
Probes
Science
Science (multidisciplinary)
Selectivity
United Kingdom > UK
Spain
ISSN:2041-1723, 2041-1723
Online Access:Get full text
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Description
Summary:Fluorescent antimicrobial peptides are promising structures for in situ , real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity. Functionalizing antimicrobial peptides with fluorescent groups is a useful strategy for imaging infection, but the tag can alter the performance of the probe. Here, the authors report a spacer-free method to directly functionalise an amino acid with a fluorogenic group and prepare peptide-based imaging agents for fungal infection.
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ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms10940