Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue

Fluorescent antimicrobial peptides are promising structures for in situ , real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage...

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Vydané v:	Nature communications Ročník 7; číslo 1; s. 10940 - 9
Hlavní autori:	Mendive-Tapia, Lorena, Zhao, Can, Akram, Ahsan R., Preciado, Sara, Albericio, Fernando, Lee, Martin, Serrels, Alan, Kielland, Nicola, Read, Nick D, Lavilla, Rodolfo, Vendrell, Marc
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 09.03.2016 Nature Publishing Group Nature Portfolio
Predmet:	14 14/19 14/34 14/69 639/638/11/874 639/638/403 639/638/92/611 639/766/930/2735 Amino acids Antifungal Agents - chemistry Antifungal Agents - pharmacology Antiinfectives and antibacterials Antimicrobial peptides Aspergillosis - diagnosis Aspergillosis - microbiology Aspergillus fumigatus - chemistry Aspergillus fumigatus - drug effects Aspergillus fumigatus - physiology Bacterial infections Boron Compounds - chemistry Diagnostic Imaging - instrumentation Diagnostic Imaging - methods Fluorescence Fluorescent Dyes - chemistry Fungal infections Fungi Human performance Human tissues Humanities and Social Sciences Humans Hydrophobicity Imaging Labeling Lung - microbiology Medical research Microenvironments multidisciplinary Organic chemistry Peptides Peptides - chemistry Peptides - pharmacology Permeability Probes Science Science (multidisciplinary) Selectivity United Kingdom > UK Spain
ISSN:	2041-1723, 2041-1723
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Shrnutí:	Fluorescent antimicrobial peptides are promising structures for in situ , real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity. Functionalizing antimicrobial peptides with fluorescent groups is a useful strategy for imaging infection, but the tag can alter the performance of the probe. Here, the authors report a spacer-free method to directly functionalise an amino acid with a fluorogenic group and prepare peptide-based imaging agents for fungal infection.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2041-1723 2041-1723
DOI:	10.1038/ncomms10940