Pro- and anti-inflammatory responses of peripheral blood mononuclear cells induced by Staphylococcus aureus and Pseudomonas aeruginosa phages

The ability of bacteriophages to kill bacteria is well known, as is their potential use as alternatives to antibiotics. As such, bacteriophages reach high doses locally through infection of their bacterial host in the human body. In this study we assessed the gene expression profile of peripheral bl...

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Veröffentlicht in:Scientific reports Jg. 7; H. 1; S. 8004 - 13
Hauptverfasser: Van Belleghem, Jonas D., Clement, Frédéric, Merabishvili, Maya, Lavigne, Rob, Vaneechoutte, Mario
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 14.08.2017
Nature Publishing Group
Nature Portfolio
Schlagworte:
13/21
38/39
38/90
38/91
45/77
631/250/262
631/326/1321
631/326/596/2557
Antibiotics
Bacteriophages - immunology
Bacteriophages - pathogenicity
CD14 antigen
Cells, Cultured
Cytokines - genetics
Cytokines - metabolism
Endotoxins
Gene expression
Humanities and Social Sciences
Humans
Immune response
Immunity, Innate - genetics
Inflammation
Inflammation - genetics
Interleukin 1
Interleukin 10
Interleukin 6
Leukocytes (mononuclear)
Lipopolysaccharide Receptors - genetics
Lipopolysaccharides - immunology
Monocytes
Monocytes - immunology
Monocytes - microbiology
Monocytes - virology
multidisciplinary
Peripheral blood mononuclear cells
Phages
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas aeruginosa - virology
Science
Science (multidisciplinary)
Staphylococcus aureus
Staphylococcus aureus - immunology
Staphylococcus aureus - virology
Tumor necrosis factor-α
ISSN:2045-2322, 2045-2322
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Zusammenfassung:The ability of bacteriophages to kill bacteria is well known, as is their potential use as alternatives to antibiotics. As such, bacteriophages reach high doses locally through infection of their bacterial host in the human body. In this study we assessed the gene expression profile of peripheral blood monocytes from six donors for twelve immunity-related genes ( i . e . CD14 , CXCL1 , CXCL5 , IL1A , IL1B , IL1RN , IL6 , IL10 , LYZ , SOCS3 , TGFBI and TNFA ) induced by Staphylococcus aureus phage ISP and four Pseudomonas aeruginosa phages ( i . e . PNM, LUZ19, 14-1 and GE-vB_Pae-Kakheti25). The phages were able to induce clear and reproducible immune responses. Moreover, the overall immune response was very comparable for all five phages: down-regulation of LYZ and TGFBI , and up-regulation of CXCL1 , CXCL5 , IL1A , IL1B , IL1RN , IL6 , SOCS3 and TNFA . The observed immune response was shown to be endotoxin-independent and predominantly anti-inflammatory. Addition of endotoxins to the highly purified phages did not cause an immune response comparable to the one induced by the (endotoxin containing) phage lysate. In addition, the use of an intermediate level of endotoxins tipped the immune response to a more anti-inflammatory response, i . e . up-regulation of IL1RN and a strongly reduced expression of CXCL1 and CXCL5.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-08336-9