Pro- and anti-inflammatory responses of peripheral blood mononuclear cells induced by Staphylococcus aureus and Pseudomonas aeruginosa phages

The ability of bacteriophages to kill bacteria is well known, as is their potential use as alternatives to antibiotics. As such, bacteriophages reach high doses locally through infection of their bacterial host in the human body. In this study we assessed the gene expression profile of peripheral bl...

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Vydané v:	Scientific reports Ročník 7; číslo 1; s. 8004 - 13
Hlavní autori:	Van Belleghem, Jonas D., Clement, Frédéric, Merabishvili, Maya, Lavigne, Rob, Vaneechoutte, Mario
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 14.08.2017 Nature Publishing Group Nature Portfolio
Predmet:	13/21 38/39 38/90 38/91 45/77 631/250/262 631/326/1321 631/326/596/2557 Antibiotics Bacteriophages - immunology Bacteriophages - pathogenicity CD14 antigen Cells, Cultured Cytokines - genetics Cytokines - metabolism Endotoxins Gene expression Humanities and Social Sciences Humans Immune response Immunity, Innate - genetics Inflammation Inflammation - genetics Interleukin 1 Interleukin 10 Interleukin 6 Leukocytes (mononuclear) Lipopolysaccharide Receptors - genetics Lipopolysaccharides - immunology Monocytes Monocytes - immunology Monocytes - microbiology Monocytes - virology multidisciplinary Peripheral blood mononuclear cells Phages Pseudomonas aeruginosa Pseudomonas aeruginosa - immunology Pseudomonas aeruginosa - virology Science Science (multidisciplinary) Staphylococcus aureus Staphylococcus aureus - immunology Staphylococcus aureus - virology Tumor necrosis factor-α
ISSN:	2045-2322, 2045-2322
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Shrnutí:	The ability of bacteriophages to kill bacteria is well known, as is their potential use as alternatives to antibiotics. As such, bacteriophages reach high doses locally through infection of their bacterial host in the human body. In this study we assessed the gene expression profile of peripheral blood monocytes from six donors for twelve immunity-related genes ( i . e . CD14 , CXCL1 , CXCL5 , IL1A , IL1B , IL1RN , IL6 , IL10 , LYZ , SOCS3 , TGFBI and TNFA ) induced by Staphylococcus aureus phage ISP and four Pseudomonas aeruginosa phages ( i . e . PNM, LUZ19, 14-1 and GE-vB_Pae-Kakheti25). The phages were able to induce clear and reproducible immune responses. Moreover, the overall immune response was very comparable for all five phages: down-regulation of LYZ and TGFBI , and up-regulation of CXCL1 , CXCL5 , IL1A , IL1B , IL1RN , IL6 , SOCS3 and TNFA . The observed immune response was shown to be endotoxin-independent and predominantly anti-inflammatory. Addition of endotoxins to the highly purified phages did not cause an immune response comparable to the one induced by the (endotoxin containing) phage lysate. In addition, the use of an intermediate level of endotoxins tipped the immune response to a more anti-inflammatory response, i . e . up-regulation of IL1RN and a strongly reduced expression of CXCL1 and CXCL5.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-017-08336-9