Loss of H3K27me3 imprinting in the Sfmbt2 miRNA cluster causes enlargement of cloned mouse placentas

Somatic cell nuclear transfer (SCNT) in mammals is an inefficient process that is frequently associated with abnormal phenotypes, especially in placentas. Recent studies demonstrated that mouse SCNT placentas completely lack histone methylation (H3K27me3)-dependent imprinting, but how it affects pla...

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Vydané v:Nature communications Ročník 11; číslo 1; s. 2150 - 12
Hlavní autori: Inoue, Kimiko, Ogonuki, Narumi, Kamimura, Satoshi, Inoue, Hiroki, Matoba, Shogo, Hirose, Michiko, Honda, Arata, Miura, Kento, Hada, Masashi, Hasegawa, Ayumi, Watanabe, Naomi, Dodo, Yukiko, Mochida, Keiji, Ogura, Atsuo
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 01.05.2020
Nature Publishing Group
Nature Portfolio
Predmet:
38/1
38/39
38/61
631/136/2435
631/337/176/1968
631/337/384
64/60
Animals
Birth
Birth rate
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Cloning
Embryos
Enlargement
Epigenetics
Expansion
Female
Gene expression
Genes
Genomic Imprinting
Histology
Histones
Histones - metabolism
Humanities and Social Sciences
Imprinting
Methylation
Mice
MicroRNAs - genetics
miRNA
multidisciplinary
Multigene Family - genetics
Nuclear transfer
Phenotypes
Placenta
Placenta - metabolism
Pregnancy
Repressor Proteins - genetics
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
Science
Science (multidisciplinary)
Somatic cell nuclear transfer
ISSN:2041-1723, 2041-1723
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Popis
Shrnutí:Somatic cell nuclear transfer (SCNT) in mammals is an inefficient process that is frequently associated with abnormal phenotypes, especially in placentas. Recent studies demonstrated that mouse SCNT placentas completely lack histone methylation (H3K27me3)-dependent imprinting, but how it affects placental development remains unclear. Here, we provide evidence that the loss of H3K27me3 imprinting is responsible for abnormal placental enlargement and low birth rates following SCNT, through upregulation of imprinted miRNAs. When we restore the normal paternal expression of H3K27me3-dependent imprinted genes ( Sfmbt2 , Gab1 , and Slc38a4 ) in SCNT placentas by maternal knockout, the placentas remain enlarged. Intriguingly, correcting the expression of clustered miRNAs within the Sfmbt2 gene ameliorates the placental phenotype. Importantly, their target genes, which are confirmed to cause SCNT-like placental histology, recover their expression level. The birth rates increase about twofold. Thus, we identify loss of H3K27me3 imprinting as an epigenetic error that compromises embryo development following SCNT. Somatic cell nuclear transfer (SCNT) frequently results in abnormal placenta development in cloned mice. Here the authors show that loss of histone methylation (H3K27me3) imprinting in clustered Sfmbt2 miRNAs contributes to SCNT placenta defect.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-020-16044-8