A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels

Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Biofabrication Ročník 11; číslo 2; s. 025014
Hlavní autori: Ko, Hyojin, Suthiwanich, Kasinan, Mary, Héloïse, Zanganeh, Somayeh, Hu, Shu-Kai, Ahadian, Samad, Yang, Yunzhi, Choi, Goro, Fetah, Kirsten, Niu, Yuting, Mao, Jeremy J, Khademhosseini, Ali
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England 28.03.2019
Predmet:
Animals
Cross-Linking Reagents - chemistry
Humans
Hydrogels - chemistry
Light
Mechanical Phenomena
Methacrylates - chemistry
Mice
NIH 3T3 Cells
Polymers - chemistry
Serum Albumin, Bovine - metabolism
Swine
Ultraviolet Rays
ISSN:1758-5090, 1758-5090
On-line prístup:Zistit podrobnosti o prístupe
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly desirable. This work demonstrates a technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels by stacking and crosslinking prehydrogel solution in a layer by layer manner. Partial crosslinking of the hydrogel allows mixing of prehydrogel solution with the previous hydrogel layer, which makes a smooth gradient profile, rather than discrete layers. This technique enables the generation of concentration gradients of bovine serum albumin in both gelatin methacryloyl (GelMA) and poly(ethylene glycol) diacrylate hydrogels, as well as mechanical gradients across a hydrogel containing varying gel concentrations. Fluorescence microscopy, mechanical testing, and scanning electron microscopy show that the gradient profiles can be controlled by changing both the volume and concentration of each layer as well as intensity of UV exposure. GelMA hydrogel gradients with different Young's moduli were successfully used to culture human fibroblasts. The fibroblasts migrated along the gradient axis and showed different morphologies. In general, the proposed technique provides a rapid and simple approach to design and fabricate 3D hydrogel gradients for in vitro biological studies and potentially for in vivo tissue engineering applications.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1758-5090
1758-5090
DOI:10.1088/1758-5090/ab08b5