Membrane platforms for biological nanopore sensing and sequencing

[Display omitted] •Biological nanopores enable label-free single molecule sensing.•Nanopores have great potential for low cost rapid DNA sequencing.•Improved artificial membrane platforms enable nanopore devices and applications. In the past two decades, biological nanopores have been developed and...

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Bibliographic Details
Published in:Current opinion in biotechnology Vol. 39; pp. 17 - 27
Main Author: Schmidt, Jacob
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.06.2016
Subjects:
artificial membranes
Biosensing Techniques - methods
biotechnology
genes
Internal Medicine
Membranes, Artificial
Nanopores
nucleic acids
polymers
Proteins - chemistry
Sequence Analysis, DNA
Sequence Analysis, Protein
ISSN:0958-1669, 1879-0429, 1879-0429
Online Access:Get full text
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Summary:[Display omitted] •Biological nanopores enable label-free single molecule sensing.•Nanopores have great potential for low cost rapid DNA sequencing.•Improved artificial membrane platforms enable nanopore devices and applications. In the past two decades, biological nanopores have been developed and explored for use in sensing applications as a result of their exquisite sensitivity and easily engineered, reproducible, and economically manufactured structures. Nanopore sensing has been shown to differentiate between highly similar analytes, measure polymer size, detect the presence of specific genes, and rapidly sequence nucleic acids translocating through the pore. Devices featuring protein nanopores have been limited in part by the membrane support containing the nanopore, the shortcomings of which have been addressed in recent work developing new materials, approaches, and apparatus resulting in membrane platforms featuring automatability and increased robustness, lifetime, and measurement throughput.
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ISSN:0958-1669
1879-0429
1879-0429
DOI:10.1016/j.copbio.2015.12.015