Ultrastructural features of hepatocytes in cultured Eurasian perch (Perca fluviatilis L.) as affected by nutritional and husbandry conditions

A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to quantify different hepatocyte ultrastructural features as potentially influenced by twelve nutritional and husbandry factors, in order to discr...

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Vydáno v:	British journal of nutrition Ročník 100; číslo 2; s. 317 - 331
Hlavní autoři:	Blanchard, Gersande, Gardeur, Jean N., Mathis, Nicolas, Brun-Bellut, Jean, Kestemont, Patrick
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Cambridge, UK Cambridge University Press 01.08.2008 Cambridge University Press (CUP)
Témata:	analysis Animal Feed Animal Feed - analysis animal nutrition Animal Nutritional Physiological Phenomena Animals Aquaculture astaxanthin Biological and medical sciences body weight chemistry Diet dietary fat dietary protein endoplasmic reticulum Factorial experiments farmed fish fatty liver feed rations Feeding. Feeding behavior Female fish farms fish feeding Fish oils Fisheries Fisheries - methods Food and Nutrition Fractional factorial design freshwater fish Fundamental and applied biological sciences. Psychology glycogen growth & development hepatocytes Hepatocytes - chemistry Hepatocytes - ultrastructure Heterogeneity Life Sciences light spectrum Lipids Lipids - analysis Liver Metabolism methods Microscopy, Electron mitochondria Nutrition research Nutritional and husbandry factors Perca fluviatilis Perches Perches - growth & development Perches - physiology Photoperiod physiology Proteins rough endoplasmic reticulum stocking rate Temperature Ultrastructural features of hepatocytes ultrastructure Vertebrates: anatomy and physiology, studies on body, several organs or systems water temperature France Fractional factorial design Ultrastructural features of hepatocytes Perca fluviatilis Nutritional and husbandry factors Cell culture Vertebrata Mammalia Hepatocyte Nutrition Perca fluviatilis: Ultrastructural features of hepatocytes: Nutritional and husbandry factors: Fractional factorial design Digestive system Ultrastructure Liver CONDITIONS D'ELEVAGE NUTTRITIONAL FACTORS FRACTIONAL FACTORIAL DESIGN PERCA FLUVIATILIS HUSBANDRY FACTORS ULTRASTRUCTURAL FEATURES OF HEPATOCYTES
ISSN:	0007-1145, 1475-2662, 1475-2662
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Shrnutí:	A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to quantify different hepatocyte ultrastructural features as potentially influenced by twelve nutritional and husbandry factors, in order to discriminate the most influent factors in Eurasian perch (Perca fluviatilis), a typical carnivorous temperate fish species. Twenty-four groups of juveniles (initial weight 57·6 (sd 14·4) g) were intensively reared for 116 d and fed sixteen different isoproteic diets. The distribution of the experimental treatments was based on a multivariate fractional factorial design (L24 212) with either high (+1) or low ( − 1) level of each of the following factors: diet (lipid and protein sources, lipid content, astaxanthin enrichment), feeding level, daily and weekly distribution frequency, fish density, initial weight heterogeneity, temperature, photoperiod, and light spectrum. Liver lipid droplets, glycogen, mitochondria and rough endoplasmic reticulum (RER) were semi-quantified and analysed by a soft imaging system using transmission electronic microscopy photographs. Important variability of hepatocyte ultrastructural features was observed. The present study confirms that the rearing temperature, through its influences in the general metabolic activity, seems to be the main factor modifying mainly lipid droplet accumulation and RER development. However, factors that could be pooled under the designation of factors leading to food accessibility and lipid and protein quality intensify or compensate the effect of temperature.
Bibliografie:	ArticleID:92653 istex:2567DB49860CC636993CA10C13FEF3424B1F439B PII:S0007114508926532 ark:/67375/6GQ-PM5VHSFV-0 Abbreviations: agly, area of glycogen per micrograph; ald, mean area of lipid droplet per micrograph; amit, mean area of mitochondria per micrograph; arer, area of rough endoplasmic reticulum per micrograph; Ast, astaxanthin enrichment; IG, gonadosomatic index; IH, hepatosomatic index; LSF, fish oil as the lipid source; nld, number of lipid droplets per micrograph; PSF, pure fishmeal as the protein source; PSFV, fish and vegetable meal as the protein source; RER, rough endoplasmic reticulum; tld, total area of lipid droplets per micrograph ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23
ISSN:	0007-1145 1475-2662 1475-2662
DOI:	10.1017/S0007114508926532