One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

•We describe three long-read sequencing technologies and their characteristics.•We describe available algorithms for long-read assembly and their use.•Most microbial genomes can now be automatically finished using long reads.•The total cost of finishing microbial genomes is now under $1000.•New meth...

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Bibliographic Details

Published in:	Current opinion in microbiology Vol. 23; pp. 110 - 120
Main Authors:	Koren, Sergey, Phillippy, Adam M
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01.02.2015
Subjects:	automation Chromosomes Contig Mapping - methods Contig Mapping - trends genome Genome, Microbial Molecular Biology - methods Molecular Biology - trends Pathology Sequence Analysis, DNA - methods Sequence Analysis, DNA - trends
ISSN:	1369-5274, 1879-0364, 1879-0364
Online Access:	Get full text
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