Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families

Domestic cats are commonly affected by viral pathogens that induce lengthy infections with fatal outcomes. Prevention of viral propagation is of primordial importance in shelters and catteries, where cats from different backgrounds have narrow contacts. Oligonucleotides (ODN) containing cytosine-pho...

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Vydáno v:	Veterinary research (Paris) Ročník 43; číslo 1; s. 60 - 197
Hlavní autoři:	Robert-Tissot, Céline, Rüegger, Vera L, Cattori, Valentino, Meli, Marina L, Riond, Barbara, Moore, Peter F, Engels, Monika, Franchini, Marco, Hofmann-Lehmann, Regina, Lutz, Hans
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 20.08.2012 BioMed Central Ltd BMC
Témata:	Animals B cells Caliciviridae Cat Diseases - immunology Cat Diseases - prevention & control Cat Diseases - virology Cats Cell Line Cell Proliferation Coronaviridae epithelial cells Feline leukemia virus Flow Cytometry - veterinary Gene expression Gene Expression Regulation gene targeting Genetic aspects Health aspects Herpesviridae Immune response immunity Immunity, Innate Immunology Infection Leukocytes, Mononuclear - immunology Male Medicine Medicine & Public Health Microbiology mononuclear leukocytes Myxovirus Resistance Proteins - genetics Myxovirus Resistance Proteins - metabolism Oligodeoxyribonucleotides - administration & dosage Oligodeoxyribonucleotides - pharmacology Oligonucleotides Parvoviridae pathogens Phosphates Physiological aspects Random Allocation Real-Time Polymerase Chain Reaction - veterinary Retroviridae Species Specificity subcutaneous injection Veterinary Medicine/Veterinary Science Virology Virus Diseases - immunology Virus Diseases - prevention & control Virus Diseases - veterinary Virus Diseases - virology Virus Replication viruses Viruses - classification Viruses - immunology Switzerland Feline Cell Viral Inhibition Assay Vesicular Stomatitis Virus Proviral Load CrFK Cell
ISSN:	1297-9716, 0928-4249, 1297-9716
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Shrnutí:	Domestic cats are commonly affected by viral pathogens that induce lengthy infections with fatal outcomes. Prevention of viral propagation is of primordial importance in shelters and catteries, where cats from different backgrounds have narrow contacts. Oligonucleotides (ODN) containing cytosine-phosphate-guanosine motifs of class A (CpG-A) are highly potent synthetic inducers of innate antiviral mechanisms. The aim of this study was to test their ability to modulate innate immune responses and prevent viral replication as stand-alone agents in the domestic cat. CpG-A stimulation of feline peripheral blood mononuclear cells (PBMCs) enhanced their proliferation, increased the presence of co-stimulatory molecules on their surface and influenced their gene expression profiles in an antiviral orientation. Incubation of the supernatants of CpG-A stimulated PBMCs with feline cell lines of epithelial and fibroblastic origin induced expression of the antiviral myxovirus resistance (Mx) gene in these target cells, which also showed enhanced resistance to feline viruses from five distinct families, namely Coronaviridae , Herpesviridae , Caliciviridae , Parvoviridae , and Retroviridae . Most importantly, subcutaneous administration of CpG-A in domestic cats systemically increased the expression of Mx, reaching maximal levels within 24 h. Plasma from treated cats could furthermore inhibit viral replication in vitro . Altogether, our data highlight the promising potential of CpG-A to induce a preventive antiviral state in the cat and to protect feline populations against a broad range of virus infections.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1297-9716 0928-4249 1297-9716
DOI:	10.1186/1297-9716-43-60