Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families

Domestic cats are commonly affected by viral pathogens that induce lengthy infections with fatal outcomes. Prevention of viral propagation is of primordial importance in shelters and catteries, where cats from different backgrounds have narrow contacts. Oligonucleotides (ODN) containing cytosine-pho...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary research (Paris) Vol. 43; no. 1; pp. 60 - 197
Main Authors: Robert-Tissot, Céline, Rüegger, Vera L, Cattori, Valentino, Meli, Marina L, Riond, Barbara, Moore, Peter F, Engels, Monika, Franchini, Marco, Hofmann-Lehmann, Regina, Lutz, Hans
Format: Journal Article
Language:English
Published: London BioMed Central 20.08.2012
BioMed Central Ltd
BMC
Subjects:
Animals
B cells
Caliciviridae
Cat Diseases - immunology
Cat Diseases - prevention & control
Cat Diseases - virology
Cats
Cell Line
Cell Proliferation
Coronaviridae
epithelial cells
Feline leukemia virus
Flow Cytometry - veterinary
Gene expression
Gene Expression Regulation
gene targeting
Genetic aspects
Health aspects
Herpesviridae
Immune response
immunity
Immunity, Innate
Immunology
Infection
Leukocytes, Mononuclear - immunology
Male
Medicine
Medicine & Public Health
Microbiology
mononuclear leukocytes
Myxovirus Resistance Proteins - genetics
Myxovirus Resistance Proteins - metabolism
Oligodeoxyribonucleotides - administration & dosage
Oligodeoxyribonucleotides - pharmacology
Oligonucleotides
Parvoviridae
pathogens
Phosphates
Physiological aspects
Random Allocation
Real-Time Polymerase Chain Reaction - veterinary
Retroviridae
Species Specificity
subcutaneous injection
Veterinary Medicine/Veterinary Science
Virology
Virus Diseases - immunology
Virus Diseases - prevention & control
Virus Diseases - veterinary
Virus Diseases - virology
Virus Replication
viruses
Viruses - classification
Viruses - immunology
Switzerland
Feline Cell
Viral Inhibition Assay
Vesicular Stomatitis Virus
Proviral Load
CrFK Cell
ISSN:1297-9716, 0928-4249, 1297-9716
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Domestic cats are commonly affected by viral pathogens that induce lengthy infections with fatal outcomes. Prevention of viral propagation is of primordial importance in shelters and catteries, where cats from different backgrounds have narrow contacts. Oligonucleotides (ODN) containing cytosine-phosphate-guanosine motifs of class A (CpG-A) are highly potent synthetic inducers of innate antiviral mechanisms. The aim of this study was to test their ability to modulate innate immune responses and prevent viral replication as stand-alone agents in the domestic cat. CpG-A stimulation of feline peripheral blood mononuclear cells (PBMCs) enhanced their proliferation, increased the presence of co-stimulatory molecules on their surface and influenced their gene expression profiles in an antiviral orientation. Incubation of the supernatants of CpG-A stimulated PBMCs with feline cell lines of epithelial and fibroblastic origin induced expression of the antiviral myxovirus resistance (Mx) gene in these target cells, which also showed enhanced resistance to feline viruses from five distinct families, namely Coronaviridae , Herpesviridae , Caliciviridae , Parvoviridae , and Retroviridae . Most importantly, subcutaneous administration of CpG-A in domestic cats systemically increased the expression of Mx, reaching maximal levels within 24 h. Plasma from treated cats could furthermore inhibit viral replication in vitro . Altogether, our data highlight the promising potential of CpG-A to induce a preventive antiviral state in the cat and to protect feline populations against a broad range of virus infections.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1297-9716
0928-4249
1297-9716
DOI:10.1186/1297-9716-43-60