Derivatization of estrogens enhances specificity and sensitivity of analysis of human plasma and serum by liquid chromatography tandem mass spectrometry

Estrogens circulate at concentrations less than 20pg/mL in men and postmenopausal women, presenting analytical challenges. Quantitation by immunoassay is unreliable at these low concentrations. Liquid chromatography tandem mass spectrometry (LC–MS/MS) offers greater specificity and sometimes greater...

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Bibliographic Details
Published in:	Talanta (Oxford) Vol. 151; pp. 148 - 156
Main Authors:	Faqehi, Abdullah M.M., Cobice, Diego F., Naredo, Gregorio, Mak, Tracy C.S., Upreti, Rita, Gibb, Fraser W., Beckett, Geoffrey J., Walker, Brian R., Homer, Natalie Z.M., Andrew, Ruth
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.05.2016 Elsevier
Subjects:	Adult Aged Aged, 80 and over Benzenesulfonates - chemistry blood serum Chromatography, Liquid - methods Derivatives Derivatization detection limit Estradiol Estradiol - blood Estradiol - chemistry Estradiol - isolation & purification Estrogens Estrogens - blood Estrogens - chemistry Estrogens - isolation & purification Estrone Estrone - blood Estrone - chemistry Estrone - isolation & purification Female Females Human Humans immunoassays Ionization ions Liquid chromatography Male males Mass spectrometry men Methylpyridinium ether Middle Aged moieties monitoring Postmenopause premenopause Premenopause - blood Pyridinium Compounds - chemistry quantitative analysis Reproducibility of Results Serums Solid Phase Extraction spectrometers Spectrometry, Mass, Electrospray Ionization tandem mass spectrometry Tandem Mass Spectrometry - methods women Young Adult 17αE2 D MRM SPE 3,4-[13C2E1 APCI E1 Derivatization E2 Estradiol LC-MS/MS LODs SD SNR MD ESI Methylpyridinium ether UHPLC HPLC CID RSD LOQs cps IS Liquid chromatography RME TEA d4E1 LC-MS d4E2 13C2E2 FMP-TS Estrone FA Mass spectrometry
ISSN:	0039-9140, 1873-3573, 1873-3573
Online Access:	Get full text
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