Characterization of isomeric glycan structures by LC‐MS/MS

The characterization of glycosylation is critical for obtaining a comprehensive view of the regulation and functions of glycoproteins of interest. Due to the complex nature of oligosaccharides, stemming from variable compositions and linkages, and ion suppression effects, the chromatographic separat...

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Vydáno v:Electrophoresis Ročník 38; číslo 17; s. 2100 - 2114
Hlavní autoři: Veillon, Lucas, Huang, Yifan, Peng, Wenjing, Dong, Xue, Cho, Byeong Gwan, Mechref, Yehia
Médium: Journal Article
Jazyk:angličtina
Vydáno: Germany Wiley Subscription Services, Inc 01.09.2017
Témata:
carbon
chromatography
Chromatography, Liquid - methods
electrophoresis
Glycan
Glycan analysis
glycomics
glycoproteins
glycosylation
Graphitization
ion exchange
Isomeric separation
Isomerism
LC‐MS/MS
Linkages
Liquid chromatography
Mass spectrometry
Oligosaccharides
polysaccharides
Polysaccharides - analysis
Polysaccharides - chemistry
researchers
Tandem Mass Spectrometry - methods
Glycan analysis
LC-MS/MS
Liquid chromatography
Mass spectrometry
Isomeric separation
ISSN:0173-0835, 1522-2683, 1522-2683
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Popis
Shrnutí:The characterization of glycosylation is critical for obtaining a comprehensive view of the regulation and functions of glycoproteins of interest. Due to the complex nature of oligosaccharides, stemming from variable compositions and linkages, and ion suppression effects, the chromatographic separation of glycans, including isomeric structures, is necessary for exhaustive characterization by MS. This review introduces the fundamental principles underlying the techniques in LC utilized by modern day glycomics researchers. Recent advances in porous graphitized carbon, reverse phase, ion exchange, and hydrophilic interaction LC utilized in conjunction with MS, for the characterization of protein glycosylation, are described with an emphasis on methods capable of resolving isomeric glycan structures.
Bibliografie:These authors contributed equally.
See the article online to view Figs. 1, 3, 5–10 in colour.
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authors contributed equally
ISSN:0173-0835
1522-2683
1522-2683
DOI:10.1002/elps.201700042