Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION -3.1

Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be use...

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Bibliographic Details
Published in:IUCrJ Vol. 7; no. 2; pp. 253 - 267
Main Authors: Zivanov, Jasenko, Nakane, Takanori, Scheres, Sjors H. W.
Format: Journal Article
Language:English
Published: England International Union of Crystallography 01.03.2020
Subjects:
Aberration
aberrations
Algorithms
Analysis
anisotropic magnification
Anisotropy
cryo-em
Datasets
Detection equipment
Microscopy
Open source software
Optical equipment
Radiation (Physics)
Reconstruction
relion
Research Papers
Source code
Technology
anisotropic magnification
aberrations
RELION
cryo-EM
ISSN:2052-2525, 2052-2525
Online Access:Get full text
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Summary:Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be used to correct for these aberrations for any given cryo-EM data set, a posteriori . Using five publicly available data sets, it is shown that considering these aberrations improves the resolution of the 3D reconstruction when these effects are present. The methods are implemented in version 3.1 of the open-source software package RELION .
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ISSN:2052-2525
2052-2525
DOI:10.1107/S2052252520000081