Depot-specific differences in inflammatory mediators and a role for NK cells and IFN-gamma in inflammation in human adipose tissue

Background: Adipose tissue is a primary in vivo site of inflammation in obesity. Excess visceral adipose tissue (VAT), when compared to subcutaneous adipose tissue (SAT), imparts an increased risk of obesity-related comorbidities and mortality, and exhibits differences in inflammation. Defining depo...

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Vydáno v:	International Journal of Obesity Ročník 33; číslo 9; s. 978 - 990
Hlavní autoři:	O'Rourke, R.W, Metcalf M.D, White, A.E, Madala, A, Winters, B.R, Maizlin, I.I, Jobe, B.A, Roberts, C.T. Jr, Slifka, M.K, Marks, D.L
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.09.2009 Nature Publishing Group
Témata:	adipose tissue Adipose tissues Adult Bariatric Surgery Biochemical assays Biological and medical sciences cytokines Diagnosis enzyme-linked immunosorbent assay Epidemiology Female flow cytometry Gene Expression Regulation Genetic transcription genetics Health Promotion and Disease Prevention Humans Immunohistochemistry in vitro studies Inflammation Inflammation Mediators Inflammation Mediators - metabolism interferon-gamma Interferon-gamma - genetics Interferon-gamma - metabolism interferons Internal Medicine Intra-Abdominal Fat Intra-Abdominal Fat - metabolism Killer Cells, Natural Killer Cells, Natural - metabolism macrophages Medical sciences Medicine Medicine & Public Health Metabolic Diseases metabolism Methods morbidity mortality natural killer cells Obesity Obesity - genetics Obesity - metabolism original-article Panniculitis Panniculitis - metabolism phenotype Properties Public Health stromovascular cell fractions Subcutaneous Fat Subcutaneous Fat - metabolism T-lymphocytes transcription factors women adipose tissue IFN-γ NK cells inflammation Human Obesity Adipose tissue Nutrition Nutrition disorder Metabolic diseases Inflammation Natural killer cell Mediator Nutritional status Comparative study Gamma interferon
ISSN:	0307-0565, 1476-5497, 1476-5497
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Popis
Shrnutí:	Background: Adipose tissue is a primary in vivo site of inflammation in obesity. Excess visceral adipose tissue (VAT), when compared to subcutaneous adipose tissue (SAT), imparts an increased risk of obesity-related comorbidities and mortality, and exhibits differences in inflammation. Defining depot-specific differences in inflammatory function may reveal underlying mechanisms of adipose-tissue-based inflammation. Methods: Stromovascular cell fractions (SVFs) from VAT and SAT from obese humans undergoing bariatric surgery were studied in an in vitro culture system with transcriptional profiling, flow cytometric phenotyping, enzyme-linked immunosorbent assay and intracellular cytokine staining. Results: Transcriptional profiling of SVF revealed differences in inflammatory transcript levels in VAT relative to SAT, including elevated interferon- (IFN-) transcript levels. VAT demonstrated a broad leukocytosis relative to SAT that included macrophages, T cells and natural killer (NK) cells. IFN- induced a proinflammatory cytokine expression pattern in SVF and adipose tissue macrophages (ATM). NK cells, which constitutively expressed IFN-, were present at higher frequency in VAT relative to SAT. Both T and NK cells from SVF expressed IFN- on activation, which was associated with tumor necrosis factor- expression in macrophages. Conclusion: These data suggest involvement of NK cells and IFN- in regulating ATM phenotype and function in human obesity and a potential mechanism for the adverse physiologic effects of VAT.
Bibliografie:	http://dx.doi.org/10.1038/ijo.2009.133 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0307-0565 1476-5497 1476-5497
DOI:	10.1038/ijo.2009.133