A novel biomarker TERTmRNA is applicable for early detection of hepatoma

Backgrounds We previously reported a highly sensitive method for serum human telomerase reverse transcriptase (hTERT) mRNA for hepatocellular carcinoma (HCC). α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are good markers for HCC. In this study, we verified the significance of hTERTmRNA in...

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Vydané v:	BMC gastroenterology Ročník 10; číslo 1; s. 46
Hlavní autori:	Miura, Norimasa, Osaki, Yukio, Nagashima, Miki, Kohno, Michimori, Yorozu, Kensho, Shomori, Kohei, Kanbe, Takamasa, Oyama, Kenji, Kishimoto, Yukihiro, Maruyama, Shigeo, Noma, Eijiro, Horie, Yutaka, Kudo, Masatoshi, Sakaguchi, Seigo, Hirooka, Yasuaki, Ito, Hisao, Kawasaki, Hironaka, Hasegawa, Junichi, Shiota, Goshi
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London BioMed Central 18.05.2010 BioMed Central Ltd Springer Nature B.V BMC
Predmet:	Adult Aged Aged, 80 and over Biological markers Biomarkers Biomarkers, Tumor - blood Cancer therapies Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - enzymology Carcinoma, Hepatocellular - genetics Clinical medicine Development and progression Diagnosis, Differential Disease Disease Progression Early Detection of Cancer - methods Female Gastroenterology Hepatology Hepatoma Hospitals Humans Identification and classification Immunohistochemistry Internal Medicine Liver cirrhosis Liver diseases Liver Neoplasms - diagnosis Liver Neoplasms - enzymology Liver Neoplasms - genetics Male Medical research Medicine Medicine & Public Health Messenger RNA Middle Aged Multivariate analysis Properties Research Article Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics ROC Curve Science Studies Telomerase Telomerase - blood Telomerase - genetics Young Adult Japan Decompensated Liver Cirrhosis Receiver Operator Characteristic Curve Analysis hTERT Expression Differentiation Degree Telomeric Repeat Amplification Protocol
ISSN:	1471-230X, 1471-230X
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Shrnutí:	Backgrounds We previously reported a highly sensitive method for serum human telomerase reverse transcriptase (hTERT) mRNA for hepatocellular carcinoma (HCC). α-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are good markers for HCC. In this study, we verified the significance of hTERTmRNA in a large scale multi-centered trial, collating quantified values with clinical course. Methods In 638 subjects including 303 patients with HCC, 89 with chronic hepatitis (CH), 45 with liver cirrhosis (LC) and 201 healthy individuals, we quantified serum hTERTmRNA using the real-time RT-PCR. We examined its sensitivity and specificity in HCC diagnosis, clinical significance, ROC curve analysis in comparison with other tumor markers, and its correlations with the clinical parameters using Pearson relative test and multivariate analyses. Furthermore, we performed a prospective and comparative study to observe the change of biomarkers, including hTERTmRNA in HCC patients receiving anti-cancer therapies. Results hTERTmRNA was demonstrated to be independently correlated with clinical parameters; tumor size and tumor differentiation (P < 0.001, each). The sensitivity/specificity of hTERTmRNA in HCC diagnosis showed 90.2%/85.4% for hTERT. hTERTmRNA proved to be superior to AFP, AFP-L3, and DCP in the diagnosis and underwent an indisputable change in response to therapy. The detection rate of small HCC by hTERTmRNA was superior to the other markers. Conclusions hTERTmRNA is superior to conventional tumor markers in the diagnosis and recurrence of HCC at an early stage.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23
ISSN:	1471-230X 1471-230X
DOI:	10.1186/1471-230X-10-46