Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels

Driven by new discoveries in stem-cell biology and regenerative medicine, there is broad interest in biomaterials that go beyond basic interactions with cells and tissues to actively direct and sustain cellular behavior. Keratin biomaterials have the potential to achieve these goals but have been in...

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Published in:Biomaterials Vol. 34; no. 11; pp. 2632 - 2640
Main Authors: Burnett, Luke R., Rahmany, Maria B., Richter, Jillian R., Aboushwareb, Tamer A., Eberli, Daniel, Ward, Catherine L., Orlando, Giuseppe, Hantgan, Roy R., Van Dyke, Mark E.
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01.04.2013
Subjects:
Adhesion
Advanced Basic Science
Animals
biocompatible materials
Biocompatible Materials - analysis
Biocompatible Materials - chemistry
Biomaterial
biotechnology
Blood Platelets - cytology
Blood Platelets - metabolism
Blotting, Western
Cell Adhesion
Colorimetry
crosslinking
Dentistry
drugs
Electrophoresis
Hair - chemistry
Hemostasis
Hemostatics - metabolism
Humans
hydrocolloids
Hydrogels - analysis
Hydrogels - chemistry
Integrin
Keratin
Keratins - analysis
Keratins - chemistry
liver
Mass Spectrometry
medicine
Microscopy, Confocal
Microscopy, Electron, Scanning
Platelet
Regenerative Medicine - methods
Rheology
Swine
tissue engineering
Tissue Engineering - methods
tissues
Keratin
Platelet
Biomaterial
Integrin
Hemostasis
Adhesion
ISSN:0142-9612, 1878-5905, 1878-5905
Online Access:Get full text
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Summary:Driven by new discoveries in stem-cell biology and regenerative medicine, there is broad interest in biomaterials that go beyond basic interactions with cells and tissues to actively direct and sustain cellular behavior. Keratin biomaterials have the potential to achieve these goals but have been inadequately described in terms of composition, structure, and cell-instructive characteristics. In this manuscript we describe and characterize a keratin-based biomaterial, demonstrate self-assembly of cross-linked hydrogels, investigate a cell-specific interaction that is dependent on the hydrogel structure and mediated by specific biomaterial–receptor interactions, and show one potential medical application that relies on receptor binding - the ability to achieve hemostasis in a lethal liver injury model. Keratin biomaterials represent a significant advance in biotechnology as they combine the compatibility of natural materials with the chemical flexibility of synthetic materials. These characteristics allow for a system that can be formulated into several varieties of cell-instructive biomaterials with potential uses in tissue engineering, regenerative medicine, drug and cell delivery, and trauma.
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ISSN:0142-9612
1878-5905
1878-5905
DOI:10.1016/j.biomaterials.2012.12.022