Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue

The aim of this study is to investigate the expression and localization of HSP70/90 in different tissues and explore the regulation effects of HSP70/90 at lactation period of female yaks. HSP90 mRNA was cloned from the heart samples of female yaks, Quantitative real-time (qRT-PCR), Western blotting...

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Published in:PloS one Vol. 12; no. 7; p. e0179321
Main Authors: Liu, Penggang, Yu, Sijiu, Cui, Yan, He, Junfeng, Yu, Chuan, Wen, Zexing, Pan, Yangyang, Yang, Kun, Song, Liangli, Yang, Xue
Format: Journal Article
Language:English
Published: United States Public Library of Science 17.07.2017
Public Library of Science (PLoS)
Subjects:
Adaptability
Amino acid sequence
Analysis
Animal lactation
Animals
Apostichopus japonicus
Biology and Life Sciences
Bos grunniens
Bovidae
Cancer
Cardiac muscle
Cattle
Cell division
Cerebellum
Cloning
Cloning, Molecular
Cold
Conserved sequence
Female
Females
Fibroblasts
Gene expression
Gene Expression Regulation
Genetic aspects
Heart
Heart diseases
Heat
Heat shock proteins
High temperature
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Hsp70 protein
HSP90 Heat-Shock Proteins - genetics
HSP90 Heat-Shock Proteins - metabolism
Hsp90 protein
Humans
Immune system
Immunofluorescence
Immunohistochemistry
Kinases
Lactation
Localization
Mammary Glands, Animal - metabolism
Medicine and Health Sciences
Medulla
Medulla oblongata
mRNA
Muscles
Organ Specificity
Physiological aspects
Protein expression
Protein folding
Reproduction
Reproductive system
Research and Analysis Methods
RNA, Messenger - genetics
RNA, Messenger - metabolism
Studies
Theca
Tissue analysis
Tissues
Veterinary colleges
Veterinary medicine
Western blotting
Yaks
China
ISSN:1932-6203, 1932-6203
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Summary:The aim of this study is to investigate the expression and localization of HSP70/90 in different tissues and explore the regulation effects of HSP70/90 at lactation period of female yaks. HSP90 mRNA was cloned from the heart samples of female yaks, Quantitative real-time (qRT-PCR), Western blotting (WB), immunohistochemistry and immunofluorescence assays were utilized to analyze the expressions of HSP70/90 mRNA and protein in different tissues. Sequence analysis showed that HSP90 is a conserved molecular chaperone of female yaks. The qRT-PCR, WB results showed that the expressions of HSP70/90 mRNA and protein were significantly different in different tissues, and 3-fold higher expression during the lactation period than the non-lactation period of breast tissue (P < 0.01). Immunohistochemistry and immunofluorescence assays results showed that HSP70/90 were located in the cardiac muscle cells, cerebellar medulla, theca cells lining at the reproductive system, and the mammary epithelia of the breasts. In addition, the expression level of HSP70 was higher than those of HSP90 in all examined tissues. Therefore, our results strongly suggest that the expression and localization of HSP70/90 could provide significant evidence to further research in tissue specific expression, and lactation function of female yaks.
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Competing Interests: The authors have declared that no competing interests exist.
Conceptualization: YC.Data curation: PL YC.Formal analysis: PL YC.Funding acquisition: YC.Investigation: PL SY JH CY ZW YP KY LS XY.Methodology: PL CY LS.Project administration: YC.Resources: PL YC.Software: PL.Supervision: YC.Validation: PL SY JH CY ZW YP KY LS XY.Visualization: PL YC XY.Writing – original draft: PL.Writing – review & editing: PL YC.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0179321