Identification of autoantibody biomarkers for primary Sjögren's syndrome using protein microarrays

Sjögren's syndrome (SS) is a chronic, progressive autoimmune disease primarily affecting women. Diagnosis of SS requires an invasive salivary gland tissue biopsy and a long delay from the start of the symptoms to final diagnosis has been frequently observed. In this study, we aim to identify sa...

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Bibliographic Details
Published in:Proteomics (Weinheim) Vol. 11; no. 8; pp. 1499 - 1507
Main Authors: Hu, Shen, Vissink, Arjan, Arellano, Martha, Roozendaal, Caroline, Zhou, Hui, Kallenberg, Cees G. M., Wong, David T.
Format: Journal Article
Language:English
Published: Weinheim WILEY-VCH Verlag 01.04.2011
WILEY‐VCH Verlag
Wiley-VCH
Wiley Subscription Services, Inc
Subjects:
Analytical, structural and metabolic biochemistry
Antibodies, Antinuclear - analysis
Autoantibodies - analysis
Autoantibodies - immunology
Autoantibody biomarker
Biological and medical sciences
Biomarkers - analysis
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Histones - immunology
Humans
Lupus Erythematosus, Systemic - immunology
Medical sciences
Miscellaneous
Protein Array Analysis
Protein arrays
Protein microarray
Proteins
Saliva - immunology
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Sjogren-Larsson Syndrome - diagnosis
Sjogren-Larsson Syndrome - immunology
Sjogren-Larsson Syndrome - metabolism
Sjögren's syndrome
Transglutaminases - immunology
Autoimmunity
Immunopathology
Stomatology
Autoantibody
Biological marker
Autoantibody biomarker
Autoimmune disease
Identification
Protein microarray
Sjögren syndrome
Protein
Eye disease
Protein arrays
Sjögren's syndrome
Systemic disease
Proteomics
ISSN:1615-9853, 1615-9861, 1615-9861
Online Access:Get full text
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Summary:Sjögren's syndrome (SS) is a chronic, progressive autoimmune disease primarily affecting women. Diagnosis of SS requires an invasive salivary gland tissue biopsy and a long delay from the start of the symptoms to final diagnosis has been frequently observed. In this study, we aim to identify salivary autoantibody biomarkers for primary SS (pSS) using a protein microarray approach. Immune‐response protoarrays were used to profile saliva autoantibodies from patients with pSS (n=14), patients with systemic lupus erythematosus (SLE, n=13), and healthy control subjects (n=13). We identified 24 potential autoantibody biomarkers that can discriminate patients with pSS from both patients with SLE and healthy individuals. Four saliva autoantibody biomarkers, anti‐transglutaminase, anti‐histone, anti‐SSA, and anti‐SSB, were further tested in independent pSS (n=34), SLE (n=34), and healthy control (n=34) subjects and all were successfully validated with ELISA. This study has demonstrated the potential of a high‐throughput protein microarray approach for the discovery of autoantibody biomarkers. The identified saliva autoantibody biomarkers may lead to a clinical tool for simple, noninvasive detection of pSS at low cost.
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PHS - No. R01DE017593
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istex:07E075540AAC843959C84DFD4BF792D75E5D6F49
ArticleID:PMIC201000206
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Additional corresponding author: Dr. David T. Wong, E-mail: dtww@ucla.edu
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.201000206