M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation

We show that the M2 isoform of pyruvate kinase (M2PYK) exists in equilibrium between monomers and tetramers regulated by allosteric binding of naturally occurring small-molecule metabolites. Phenylalanine stabilizes an inactive T-state tetrameric conformer and inhibits M2PYK with an IC50 value of 0....

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS Jg. 110; H. 15; S. 5881
Hauptverfasser:	Morgan, Hugh P, O'Reilly, Francis J, Wear, Martin A, O'Neill, J Robert, Fothergill-Gilmore, Linda A, Hupp, Ted, Walkinshaw, Malcolm D
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 09.04.2013
Schlagworte:	Allosteric Site Amino Acid Sequence Catalytic Domain Cell Line, Tumor Cell Proliferation Crystallography, X-Ray Dimerization Humans Inhibitory Concentration 50 Molecular Sequence Data Phenylalanine - chemistry Protein Conformation Protein Structure, Tertiary Pyruvate Kinase - metabolism Triiodothyronine - chemistry
ISSN:	1091-6490, 1091-6490
Online-Zugang:	Weitere Angaben
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