Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum

For a One-Health investigation of antimicrobial resistance (AMR) in Enterococcus spp., isolates from humans and beef cattle along with abattoirs, manured fields, natural streams, and wastewater from both urban and cattle feedlot sources were collected over two years. Species identification of Entero...

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Bibliographic Details
Published in:Scientific reports Vol. 10; no. 1; p. 3937
Main Authors: Zaheer, Rahat, Cook, Shaun R., Barbieri, Ruth, Goji, Noriko, Cameron, Andrew, Petkau, Aaron, Polo, Rodrigo Ortega, Tymensen, Lisa, Stamm, Courtney, Song, Jiming, Hannon, Sherry, Jones, Tineke, Church, Deirdre, Booker, Calvin W., Amoako, Kingsley, Van Domselaar, Gary, Read, Ron R., McAllister, Tim A.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 03.03.2020
Nature Publishing Group
Subjects:
631/326
631/326/22
631/326/22/1434
Abattoirs
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Beef cattle
beta-Lactam Resistance - genetics
Cattle
Clinical isolates
Drug resistance
Drug Resistance, Bacterial - genetics
Enterococcus
Enterococcus - drug effects
Enterococcus - genetics
Enterococcus - pathogenicity
Enterococcus faecalis - drug effects
Enterococcus faecalis - genetics
Enterococcus faecalis - pathogenicity
Enterococcus faecium - drug effects
Enterococcus faecium - genetics
Enterococcus faecium - pathogenicity
Feedlots
Genomes
Humanities and Social Sciences
Humans
Macrolides - pharmacology
multidisciplinary
Multidrug resistance
Natural streams
Phylogeny
Quinolones
Quinolones - pharmacology
Science
Science (multidisciplinary)
Species
Species diversity
Streams
Tetracyclines
Tetracyclines - pharmacology
Vancomycin
Virulence
Wastewater
Whole Genome Sequencing
β-Lactam antibiotics
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:For a One-Health investigation of antimicrobial resistance (AMR) in Enterococcus spp., isolates from humans and beef cattle along with abattoirs, manured fields, natural streams, and wastewater from both urban and cattle feedlot sources were collected over two years. Species identification of Enterococcus revealed distinct associations across the continuum. Of the 8430 isolates collected, Enterococcus faecium and Enterococcus faecalis were the main species in urban wastewater (90%) and clinical human isolates (99%); Enterococcus hirae predominated in cattle (92%) and feedlot catch-basins (60%), whereas natural streams harbored environmental Enterococcus spp. Whole-genome sequencing of E. faecalis (n = 366 isolates) and E. faecium (n = 342 isolates), revealed source clustering of isolates, indicative of distinct adaptation to their respective environments. Phenotypic resistance to tetracyclines and macrolides encoded by tet(M) and erm(B) respectively, was prevalent among Enterococcus spp. regardless of source. For E. faecium from cattle, resistance to β-lactams and quinolones was observed among 3% and 8% of isolates respectively, compared to 76% and 70% of human clinical isolates. Clinical vancomycin-resistant E. faecium exhibited high rates of multi-drug resistance, with resistance to all β-lactam, macrolides, and quinolones tested. Differences in the AMR profiles among isolates reflected antimicrobial use practices in each sector of the One-Health continuum.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-61002-5