Nitrogenase switch-off by ammonium ions in Azospirillum brasilense requires the GlnB nitrogen signal-transducing protein

Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant...

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Veröffentlicht in:Applied and environmental microbiology Jg. 71; H. 9; S. 5637
Hauptverfasser: Klassen, Giseli, Souza, Emanuel M, Yates, M Geoffrey, Rigo, Liu Un, Costa, Roberta M, Inaba, Juliana, Pedrosa, Fábio O
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.09.2005
Schlagworte:
Azospirillum brasilense - enzymology
Azospirillum brasilense - genetics
Azospirillum brasilense - growth & development
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Culture Media
Gene Expression Regulation, Bacterial
Mutation
Nitrogenase - genetics
Nitrogenase - metabolism
PII Nitrogen Regulatory Proteins
Quaternary Ammonium Compounds - pharmacology
Signal Transduction
ISSN:0099-2240
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Abstract Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.
AbstractList Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.
Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.
Author Pedrosa, Fábio O
Rigo, Liu Un
Klassen, Giseli
Inaba, Juliana
Costa, Roberta M
Yates, M Geoffrey
Souza, Emanuel M
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Snippet Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum...
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SubjectTerms Azospirillum brasilense - enzymology
Azospirillum brasilense - genetics
Azospirillum brasilense - growth & development
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Culture Media
Gene Expression Regulation, Bacterial
Mutation
Nitrogenase - genetics
Nitrogenase - metabolism
PII Nitrogen Regulatory Proteins
Quaternary Ammonium Compounds - pharmacology
Signal Transduction
Title Nitrogenase switch-off by ammonium ions in Azospirillum brasilense requires the GlnB nitrogen signal-transducing protein
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