Nitrogenase switch-off by ammonium ions in Azospirillum brasilense requires the GlnB nitrogen signal-transducing protein

Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant...

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Bibliographic Details
Published in:	Applied and environmental microbiology Vol. 71; no. 9; p. 5637
Main Authors:	Klassen, Giseli, Souza, Emanuel M, Yates, M Geoffrey, Rigo, Liu Un, Costa, Roberta M, Inaba, Juliana, Pedrosa, Fábio O
Format:	Journal Article
Language:	English
Published:	United States 01.09.2005
Subjects:	Azospirillum brasilense - enzymology Azospirillum brasilense - genetics Azospirillum brasilense - growth & development Bacterial Proteins - genetics Bacterial Proteins - metabolism Culture Media Gene Expression Regulation, Bacterial Mutation Nitrogenase - genetics Nitrogenase - metabolism PII Nitrogen Regulatory Proteins Quaternary Ammonium Compounds - pharmacology Signal Transduction
ISSN:	0099-2240
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Summary:	Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0099-2240
DOI:	10.1128/AEM.71.9.5637-5641.2005