Differential epigenetic regulation within an Arabidopsis retroposon family

We previously reported a novel family of Arabidopsis thaliana nonautonomous retroposons, Sadhu, showing epigenetic variation in natural populations. Here, we show that transcripts corresponding to Sadhu elements accumulate in a subset of mutants carrying disruptions in genes encoding chromatin modif...

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Vydáno v:	Genetics (Austin) Ročník 176; číslo 1; s. 151 - 160
Hlavní autoři:	Rangwala, S.H, Richards, E.J
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Genetics Soc America 01.05.2007 Genetics Society of America Copyright © 2007 by the Genetics Society of America
Témata:	Arabidopsis Arabidopsis - genetics Arabidopsis thaliana Base Pairing Base Pairing - genetics Chromatin Chromatin - genetics Chromatin Immunoprecipitation Chromosome Mapping cytosine Cytosine - metabolism Deoxyribonucleic acid DNA DNA Methylation Epigenesis, Genetic epigenetics gene expression Gene Expression Regulation, Plant gene silencing Genetics Genome, Plant Genome, Plant - genetics Genomics Histones Histones - metabolism Investigations Lysine Lysine - metabolism messenger RNA metabolism Methylation Molecular Sequence Data Mutants Mutation Mutation - genetics Natural populations nucleotide sequences Retroelements Retroelements - genetics retrotransposons
ISSN:	0016-6731, 1943-2631, 1943-2631
On-line přístup:	Získat plný text
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Popis
Shrnutí:	We previously reported a novel family of Arabidopsis thaliana nonautonomous retroposons, Sadhu, showing epigenetic variation in natural populations. Here, we show that transcripts corresponding to Sadhu elements accumulate in a subset of mutants carrying disruptions in genes encoding chromatin modification enzymes, but are not significantly expressed in mutants defective in RNA silencing pathways, indicating that RNA-directed processes are not necessary to maintain transcriptional suppression of this class of retroelements. We focused our analysis on three representative elements showing differential responses to ddm1, met1, and hda6 mutations. These mutations had differing effects on cytosine methylation depending on the element and the sequence context. Curiously, the Sadhu6-1 element with the strongest CpHpG methylation is expressed in a met1 CpG methyltransferase mutant, but is not expressed in ddm1 or cmt3 mutants. Regardless of the mutant background, H3meK9 was found at silenced loci, while H3meK4 was restricted to expressed alleles. We discuss the different modes of regulation within this family and the potential impact of this regulation on the stability of silencing in natural populations.
Bibliografie:	http://www.genetics.org/ ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Corresponding author: Department of Biology, Campus Box 1137, Washington University, 1 Brookings Dr., St. Louis, MO 63130. E-mail: richards@wustl.edu Communicating editor: T. P. Brutnell Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. DY230445, DY230446, EC268296, EC613982, EC613983, EC613984TH, EC613985, and EC613986.
ISSN:	0016-6731 1943-2631 1943-2631
DOI:	10.1534/genetics.107.071092