Ionic liquids modified dummy molecularly imprinted microspheres as solid phase extraction materials for the determination of clenbuterol and clorprenaline in urine

•Ionic liquid modified dummy imprinted microspheres (DMIMs) were synthesized.•DMIMs-SPE for rapid isolation of clenbuterol and clorprenaline in urine.•Matrix interferences were removed and analytes were concentrated.•Problem of template leakage on quantitative analysis was eliminated. New ionic liqu...

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Vydáno v:	Journal of Chromatography A Ročník 1294; s. 10 - 16
Hlavní autoři:	Yan, Hongyuan, Liu, Suting, Gao, Mengmeng, Sun, Ning
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Amsterdam Elsevier B.V 14.06.2013 Elsevier
Témata:	adsorbents Adsorption Analysis Analytical chemistry Biological and medical sciences Bronchodilator Agents - isolation & purification Bronchodilator Agents - urine Chemistry Chromatographic methods and physical methods associated with chromatography chromatography Clenbuterol Clenbuterol - isolation & purification Clenbuterol - urine Clorprenaline Dummies Dummy molecularly imprinted microspheres Exact sciences and technology Extraction Fourier transform infrared spectroscopy General pharmacology Humans Ionic liquids Isoproterenol - analogs & derivatives Isoproterenol - isolation & purification Isoproterenol - urine Linearity Medical sciences Microspheres Molecular Imprinting Other chromatographic methods Pharmacology. Drug treatments phenylephrine polymerization polymers Polymers - chemical synthesis Polymers - chemistry quantitative analysis Scanning electron microscopy solid phase extraction Solid Phase Extraction - instrumentation Solid Phase Extraction - methods Sorbents Urine Urine Ionic liquids Clenbuterol Clorprenaline Dummy molecularly imprinted microspheres Solid phase extraction Biological fluid Agonist Fourier transformation Chemical analysis HPLC chromatography β2-Adrenergic receptor β-Adrenergic receptor agonist Suspension copolymerization Chemical enrichment Surface structure Affinity adsorbent Characterization Methacrylic acid copolymer Sample preparation Quantitative analysis Trace analysis Human Scanning electron microscopy Microsphere Healthy subject Bronchodilator Elimination Infrared spectrometry Ultraviolet detector Morphology Preparation Allylic derivative copolymer Anabolic agent Molecular imprinting
ISSN:	0021-9673, 1873-3778, 1873-3778
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Shrnutí:	•Ionic liquid modified dummy imprinted microspheres (DMIMs) were synthesized.•DMIMs-SPE for rapid isolation of clenbuterol and clorprenaline in urine.•Matrix interferences were removed and analytes were concentrated.•Problem of template leakage on quantitative analysis was eliminated. New ionic liquid modified dummy molecularly imprinted microspheres (DMIMs) were synthesized by aqueous suspension polymerization using phenylephrine as dummy template and 1-allyl-3-ethylimidazolium bromide as co-functional monomer. The obtained DMIMs were characterized by Fourier transform infrared (FT-IR) and scanning electron microscopy (SEM), and empolyed as the special sorbent of solid-phase extraction for isolation of clenbuterol (CLB) and clorprenaline (CLP) from urine sample. The results indicated that the ionic liquid modified polymers were monodispersed microspheres and had high selectivity and adsorbability to CLB and CLP, and the matrix interferences could be efficiently eliminated from the samples. Meanwhile, the effect of template leakage of DMIMs on quantitative analysis was avoided. Compared with other commercial sorbents (HLB, PCX, C18 and SCX), the DMIMs exhibited higher affinity and purification ability to CLP and CLB. Under the optimized conditions, good linearity was observed in a range of 0.90–400μgL−1 (r2=0.9999) with LOD of 0.19 and 0.070μgL−1 for CLP and CLB, respectively. The recoveries at three spiked levels were ranged from 93.3% to 106% with RSD≤5.6% (n=3).
Bibliografie:	http://dx.doi.org/10.1016/j.chroma.2013.04.024 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9673 1873-3778 1873-3778
DOI:	10.1016/j.chroma.2013.04.024