Ionic liquids modified dummy molecularly imprinted microspheres as solid phase extraction materials for the determination of clenbuterol and clorprenaline in urine

•Ionic liquid modified dummy imprinted microspheres (DMIMs) were synthesized.•DMIMs-SPE for rapid isolation of clenbuterol and clorprenaline in urine.•Matrix interferences were removed and analytes were concentrated.•Problem of template leakage on quantitative analysis was eliminated. New ionic liqu...

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Bibliographic Details
Published in:Journal of Chromatography A Vol. 1294; pp. 10 - 16
Main Authors: Yan, Hongyuan, Liu, Suting, Gao, Mengmeng, Sun, Ning
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 14.06.2013
Elsevier
Subjects:
adsorbents
Adsorption
Analysis
Analytical chemistry
Biological and medical sciences
Bronchodilator Agents - isolation & purification
Bronchodilator Agents - urine
Chemistry
Chromatographic methods and physical methods associated with chromatography
chromatography
Clenbuterol
Clenbuterol - isolation & purification
Clenbuterol - urine
Clorprenaline
Dummies
Dummy molecularly imprinted microspheres
Exact sciences and technology
Extraction
Fourier transform infrared spectroscopy
General pharmacology
Humans
Ionic liquids
Isoproterenol - analogs & derivatives
Isoproterenol - isolation & purification
Isoproterenol - urine
Linearity
Medical sciences
Microspheres
Molecular Imprinting
Other chromatographic methods
Pharmacology. Drug treatments
phenylephrine
polymerization
polymers
Polymers - chemical synthesis
Polymers - chemistry
quantitative analysis
Scanning electron microscopy
solid phase extraction
Solid Phase Extraction - instrumentation
Solid Phase Extraction - methods
Sorbents
Urine
Urine
Ionic liquids
Clenbuterol
Clorprenaline
Dummy molecularly imprinted microspheres
Solid phase extraction
Biological fluid
Agonist
Fourier transformation
Chemical analysis
HPLC chromatography
β2-Adrenergic receptor
β-Adrenergic receptor agonist
Suspension copolymerization
Chemical enrichment
Surface structure
Affinity adsorbent
Characterization
Methacrylic acid copolymer
Sample preparation
Quantitative analysis
Trace analysis
Human
Scanning electron microscopy
Microsphere
Healthy subject
Bronchodilator
Elimination
Infrared spectrometry
Ultraviolet detector
Morphology
Preparation
Allylic derivative copolymer
Anabolic agent
Molecular imprinting
ISSN:0021-9673, 1873-3778, 1873-3778
Online Access:Get full text
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Summary:•Ionic liquid modified dummy imprinted microspheres (DMIMs) were synthesized.•DMIMs-SPE for rapid isolation of clenbuterol and clorprenaline in urine.•Matrix interferences were removed and analytes were concentrated.•Problem of template leakage on quantitative analysis was eliminated. New ionic liquid modified dummy molecularly imprinted microspheres (DMIMs) were synthesized by aqueous suspension polymerization using phenylephrine as dummy template and 1-allyl-3-ethylimidazolium bromide as co-functional monomer. The obtained DMIMs were characterized by Fourier transform infrared (FT-IR) and scanning electron microscopy (SEM), and empolyed as the special sorbent of solid-phase extraction for isolation of clenbuterol (CLB) and clorprenaline (CLP) from urine sample. The results indicated that the ionic liquid modified polymers were monodispersed microspheres and had high selectivity and adsorbability to CLB and CLP, and the matrix interferences could be efficiently eliminated from the samples. Meanwhile, the effect of template leakage of DMIMs on quantitative analysis was avoided. Compared with other commercial sorbents (HLB, PCX, C18 and SCX), the DMIMs exhibited higher affinity and purification ability to CLP and CLB. Under the optimized conditions, good linearity was observed in a range of 0.90–400μgL−1 (r2=0.9999) with LOD of 0.19 and 0.070μgL−1 for CLP and CLB, respectively. The recoveries at three spiked levels were ranged from 93.3% to 106% with RSD≤5.6% (n=3).
Bibliography:http://dx.doi.org/10.1016/j.chroma.2013.04.024
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content type line 23
ISSN:0021-9673
1873-3778
1873-3778
DOI:10.1016/j.chroma.2013.04.024