Abnormal gene expression of proinflammatory cytokines and their membrane-bound receptors in the lymphocytes of depressed patients

Abnormalities of protein levels of proinflammatory cytokines and their soluble receptors have been reported in plasma of depressed patients. In this study, we examined the role of cytokines and their membrane-bound receptors in major depressive disorder (MDD). We determined the protein and mRNA expr...

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Veröffentlicht in:	Psychiatry research Jg. 240; S. 314 - 320
Hauptverfasser:	Rizavi, Hooriyah S., Ren, Xinguo, Zhang, Hui, Bhaumik, Runa, Pandey, Ghanshyam N.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Ireland Elsevier B.V 30.06.2016
Schlagworte:	Adult Case-Control Studies Cytokines - blood Cytokines - genetics Cytokines - metabolism Depressive Disorder, Major - blood Depressive Disorder, Major - pathology Enzyme-Linked Immunosorbent Assay Female Gene Expression Humans IL-1β IL-6 Interleukin 1 Receptor Antagonist Protein - blood Interleukin-1 - blood Interleukin-1beta - blood Interleukin-1beta - metabolism Interleukin-6 - blood Lymphocytes - metabolism Major depressive disorder Male Middle Aged Polymerase Chain Reaction Psychiatry Receptors, Cytokine - genetics Receptors, Cytokine - metabolism Receptors, Interleukin-1 - blood Receptors, Interleukin-6 - blood Receptors, Tumor Necrosis Factor, Type I - blood Receptors, Tumor Necrosis Factor, Type II - blood RNA, Messenger - analysis RNA, Messenger - metabolism TNF-α Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - immunology TNFR DSM-IV IL IL-1RA CNS GLM MAP kinase IL-1R MDD IL-1β TNF BP Major depressive disorder IL-6 PRP TNF-α NC RIN Gp130 qPCR IL-6ST HDRS BMI ELISA glycoprotein130 real-time RT-PCR interleukin-1 receptor normal controls interleukin interleukin-1 receptor antagonist RNA integrity number Hamilton depression rating scale body mass index tumor necrosis factor receptor platelet-rich plasma central nervous system generalized linear model tumor necrosis factor enzyme-linked immunosorbent assay bipolar Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition Interleukin-6 signal transducer mitogen activated protein (MAP) kinase
ISSN:	0165-1781, 1872-7123
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Zusammenfassung:	Abnormalities of protein levels of proinflammatory cytokines and their soluble receptors have been reported in plasma of depressed patients. In this study, we examined the role of cytokines and their membrane-bound receptors in major depressive disorder (MDD). We determined the protein and mRNA expression of proinflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and mRNA expression of their membrane-bound receptors in the lymphocytes from 31 hospitalized MDD patients and 30 non-hospitalized normal control (NC) subjects. The subjects were diagnosed according to DSM-IV criteria. Protein levels of cytokines were determined by ELISA, and mRNA levels in lymphocytes were determined by the qPCR method. We found that the mean mRNA levels of the proinflammatory cytokines IL-1β, IL-6, TNF-α, their receptors, TNFR1, TNFR2, IL-1R1 and the antagonist IL-1RA were significantly increased in the lymphocytes of MDD patients compared with NC. No significant differences in the lymphocyte mRNA levels of IL-1R2, IL-6R, and Gp130 were observed between MDD patients and NC. These studies suggest abnormal gene expression of these cytokines and their membrane-bound receptors in the lymphocytes of MDD patients, and that their mRNA expression levels in the lymphocytes could be a useful biomarker for depression.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0165-1781 1872-7123
DOI:	10.1016/j.psychres.2016.04.049