Improvement of Silicon Nanotweezers Sensitivity for Mechanical Characterization of Biomolecules Using Closed-Loop Control

In this paper, we show that closed-loop control can be advantageously used for the characterization of mechanical properties of biomolecules using silicon nanotweezers (SNT). SNT have already been used in open-loop mode for the characterization of mechanical properties of DNA molecules. Up to now, s...

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Veröffentlicht in:IEEE/ASME Transactions on Mechatronics Jg. 20; H. 3; S. 1418 - 1427
Hauptverfasser: Lafitte, Nicolas, Haddab, Yassine, Le Gorrec, Yann, Guillou, Herve, Kumemura, Momoko, Jalabert, Laurent, Collard, Dominique, Fujita, Hiroyuki
Format: Journal Article
Sprache:Englisch
Veröffentlicht: IEEE 01.06.2015
Institute of Electrical and Electronics Engineers (IEEE)
Institute of Electrical and Electronics Engineers
Schlagworte:
[INFO.INFO-DS]Computer Science [cs]/Data Structures and Algorithms [cs.DS]
[PHYS]Physics [physics]
[SPI.AUTO]Engineering Sciences [physics]/Automatic
Automatic
Biosensor
Charge carrier processes
Computer Science
Data Structures and Algorithms
DNA
Electrodes
Engineering Sciences
MEMS tweezers
Observers
parameter detection
Resonant frequency
Sensitivity
sensitivity improvement
State feedback
MEMS tweezers
sensitivity improvement
state feedback
parameter detection
Biosensor
ISSN:1083-4435, 1941-014X
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Beschreibung
Zusammenfassung:In this paper, we show that closed-loop control can be advantageously used for the characterization of mechanical properties of biomolecules using silicon nanotweezers (SNT). SNT have already been used in open-loop mode for the characterization of mechanical properties of DNA molecules. Up to now, such an approach allows the detection of stiffness variations equivalent to about 15 DNA molecules. Here, it is shown that this resolution is inversely proportional to the resonance frequency of the whole system and that real-time feedback control with state observer can drastically improve the performances of the tweezers used as biosensors. Such improvement is experimentally validated in the case of the manipulation of fibronectin molecules. The results are promising for the accurate characterization of biopolymers such as DNA molecules.
ISSN:1083-4435
1941-014X
DOI:10.1109/TMECH.2014.2351415