Evaluation of stimulated raman scattering microscopy for identifying squamous cell carcinoma in human skin

Background and Significance There is a need to develop non‐invasive diagnostic tools to achieve early and accurate detection of skin cancer in a non‐surgical manner. In this study, we evaluate the capability of stimulated Raman scattering (SRS) microscopy, a potentially non‐invasive optical imaging...

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Published in:Lasers in surgery and medicine Vol. 45; no. 8; pp. 496 - 502
Main Authors: Mittal, Richa, Balu, Mihaela, Krasieva, Tatiana, Potma, Eric O., Elkeeb, Laila, Zachary, Christopher B., Wilder-Smith, Petra
Format: Journal Article
Language:English
Published: United States Blackwell Publishing Ltd 01.10.2013
Wiley Subscription Services, Inc
Subjects:
Carcinoma, Squamous Cell - pathology
confocal reflectance microscopy
Humans
In Vitro Techniques
Microscopy, Confocal
optical microscopy
skin cancer
Skin Neoplasms - pathology
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
squamous cell carcinoma
stimulated Raman scattering
skin cancer
stimulated Raman scattering
optical microscopy
confocal reflectance microscopy
squamous cell carcinoma
ISSN:0196-8092, 1096-9101, 1096-9101
Online Access:Get full text
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Summary:Background and Significance There is a need to develop non‐invasive diagnostic tools to achieve early and accurate detection of skin cancer in a non‐surgical manner. In this study, we evaluate the capability of stimulated Raman scattering (SRS) microscopy, a potentially non‐invasive optical imaging technique, for identifying the pathological features of s squamous cell carcinoma (SCC) tissue. Study Design We studied ex vivo SCC and healthy skin tissues using SRS microscopy, and compared the SRS contrast with the contrast obtained in reflectance confocal microscopy (RCM) and standard histology. Results and Conclusion SRS images obtained at the carbon‐hydrogen stretching vibration at 2945 cm−1 exhibit contrast related protein density that clearly delineates the cell nucleus from the cell cytoplasm. The morphological features of SCC tumor seen in the SRS images show excellent correlation with the diagnostic features identified by histological examination. Additionally, SRS exhibits enhanced cellular contrast in comparison to that seen in confocal microscopy. In conclusion, SRS represents an attractive approach for generating protein density maps with contrast that closely resembles histopathological contrast of SCC in human skin. Lasers Surg. Med. 45:496–502, 2013. © 2013 Wiley Periodicals, Inc.
Bibliography:NIH (LAMMP) - No. P41-EB015890
Air Force Office of Scientific Research AFOSR - No. FA9550-10-1-0538
istex:B244F34E5662E000EA55A453A4BB6BBB16589FF2
Chao Family Comprehensive Cancer Center and Cancer Center Support - No. P30 CA62203
ark:/67375/WNG-CX234BWL-L
ArticleID:LSM22168
American Society for Laser Medicine and Surgery ASLMS - No. S12.12
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ISSN:0196-8092
1096-9101
1096-9101
DOI:10.1002/lsm.22168