Evaluation of stimulated raman scattering microscopy for identifying squamous cell carcinoma in human skin

Background and Significance There is a need to develop non‐invasive diagnostic tools to achieve early and accurate detection of skin cancer in a non‐surgical manner. In this study, we evaluate the capability of stimulated Raman scattering (SRS) microscopy, a potentially non‐invasive optical imaging...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Lasers in surgery and medicine Jg. 45; H. 8; S. 496 - 502
Hauptverfasser:	Mittal, Richa, Balu, Mihaela, Krasieva, Tatiana, Potma, Eric O., Elkeeb, Laila, Zachary, Christopher B., Wilder-Smith, Petra
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Blackwell Publishing Ltd 01.10.2013 Wiley Subscription Services, Inc
Schlagworte:	Carcinoma, Squamous Cell - pathology confocal reflectance microscopy Humans In Vitro Techniques Microscopy, Confocal optical microscopy skin cancer Skin Neoplasms - pathology Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods squamous cell carcinoma stimulated Raman scattering skin cancer stimulated Raman scattering optical microscopy confocal reflectance microscopy squamous cell carcinoma
ISSN:	0196-8092, 1096-9101, 1096-9101
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Background and Significance There is a need to develop non‐invasive diagnostic tools to achieve early and accurate detection of skin cancer in a non‐surgical manner. In this study, we evaluate the capability of stimulated Raman scattering (SRS) microscopy, a potentially non‐invasive optical imaging technique, for identifying the pathological features of s squamous cell carcinoma (SCC) tissue. Study Design We studied ex vivo SCC and healthy skin tissues using SRS microscopy, and compared the SRS contrast with the contrast obtained in reflectance confocal microscopy (RCM) and standard histology. Results and Conclusion SRS images obtained at the carbon‐hydrogen stretching vibration at 2945 cm−1 exhibit contrast related protein density that clearly delineates the cell nucleus from the cell cytoplasm. The morphological features of SCC tumor seen in the SRS images show excellent correlation with the diagnostic features identified by histological examination. Additionally, SRS exhibits enhanced cellular contrast in comparison to that seen in confocal microscopy. In conclusion, SRS represents an attractive approach for generating protein density maps with contrast that closely resembles histopathological contrast of SCC in human skin. Lasers Surg. Med. 45:496–502, 2013. © 2013 Wiley Periodicals, Inc.
Bibliographie:	NIH (LAMMP) - No. P41-EB015890 Air Force Office of Scientific Research AFOSR - No. FA9550-10-1-0538 istex:B244F34E5662E000EA55A453A4BB6BBB16589FF2 Chao Family Comprehensive Cancer Center and Cancer Center Support - No. P30 CA62203 ark:/67375/WNG-CX234BWL-L ArticleID:LSM22168 American Society for Laser Medicine and Surgery ASLMS - No. S12.12 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 ObjectType-Undefined-3
ISSN:	0196-8092 1096-9101 1096-9101
DOI:	10.1002/lsm.22168